Ahmad Niaz, Fatima Samia, Mehmood Muhammad Aamer, Zaman Qamar U, Atif Rana Muhammad, Zhou Weijun, Rahman Mehboob-Ur, Gill Rafaqat Ali
National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, Pakistan.
Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan.
Front Plant Sci. 2023 Jun 20;14:1152468. doi: 10.3389/fpls.2023.1152468. eCollection 2023.
CRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the genome for various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, and increasing yield and quality. However, its utilization is limited to model crops for which well-annotated genome sequences are available. Many crops of dietary and economic importance, such as wheat, cotton, rapeseed-mustard, and potato, are polyploids with complex genomes. Therefore, progress in these crops has been hampered due to genome complexity. Excellent work has been conducted on some species of for its improvement through genome editing. Although excellent work has been conducted on some species of for genome improvement through editing, work on polyploid crops, including U's triangle species, holds numerous implications for improving other polyploid crops. In this review, we summarize key examples from genome editing work done on and discuss important considerations for deploying CRISPR-mediated genome editing more efficiently in other polyploid crops for improvement.
CRISPR介导的基因组编辑已成为一种强大的工具，可在基因组中创建靶向突变以用于各种应用，包括研究基因功能、培育抗生物和非生物胁迫的能力以及提高产量和品质。然而，其应用仅限于那些有注释完善的基因组序列的模式作物。许多具有饮食和经济重要性的作物，如小麦、棉花、油菜和马铃薯，都是具有复杂基因组的多倍体。因此，由于基因组复杂性，这些作物的研究进展受到了阻碍。针对某些物种通过基因组编辑进行改良的工作已经取得了出色的成果。尽管针对某些物种通过编辑进行基因组改良的工作已经取得了出色的成果，但包括U三角物种在内的多倍体作物的研究工作对于改良其他多倍体作物具有诸多启示。在本综述中，我们总结了在[此处原文缺失相关物种名称]上进行的基因组编辑工作的关键实例，并讨论了在其他多倍体作物中更有效地部署CRISPR介导的基因组编辑以进行改良的重要注意事项。
