Palomino Tana V, Muddiman David C
FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.
Mass Spectrom Rev. 2024 Jun 27. doi: 10.1002/mas.21895.
With implications in several medical conditions, N-linked glycosylation is one of the most important posttranslation modifications present in all living organisms. Due to their nontemplate synthesis, glycan structures are extraordinarily complex and require multiple analytical techniques for complete structural elucidation. Mass spectrometry is the most common way to investigate N-linked glycans; however, with techniques such as liquid-chromatography mass spectrometry, there is complete loss of spatial information. Mass spectrometry imaging is a transformative analytical technique that can visualize the spatial distribution of ions within a biological sample and has been shown to be a powerful tool to investigate N-linked glycosylation. This review covers the fundamentals of mass spectrometry imaging and N-linked glycosylation and highlights important findings of recent key studies aimed at expanding and improving the glycomics imaging field.
N-连接糖基化存在于所有生物体中，是最重要的翻译后修饰之一，与多种医学病症相关。由于聚糖结构的非模板合成，其结构极其复杂，需要多种分析技术才能完全阐明其结构。质谱是研究N-连接聚糖最常用的方法；然而，使用液相色谱质谱等技术时，会完全丢失空间信息。质谱成像技术是一种变革性的分析技术，能够可视化生物样品中离子的空间分布，已被证明是研究N-连接糖基化的有力工具。本文综述了质谱成像和N-连接糖基化的基本原理，并重点介绍了近期旨在拓展和改进糖组学成像领域的关键研究的重要发现。
