Lockridge O, La Du B N
Biochem Genet. 1986 Jun;24(5-6):485-98. doi: 10.1007/BF00499101.
Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [3H]diisopropylfluorophosphate. Peptides were purified by high-performance liquid chromatography. Amino acid composition and sequence analysis showed that the peptide from the usual genotype contained 29 residues with the sequence Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu- Leu-Ser-Pro-Gly-Ser-His-Ser-Leu-Phe-Thr-Arg. The active-site serine was the eighth residue from the N-terminal. The peptide containing the active-site serine from the atypical genotype contained 22 residues with the sequence Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu- Leu-Ser-Pro-Gly. The peptide from the atypical-silent genotype contained eight residues with the sequence Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser. Thus, the sequences of the atypical and atypical-silent active-site peptides were identical to the corresponding portions of the usual peptide.
从三种基因类型的人血清胆碱酯酶中分离出活性位点胰蛋白酶肽段。通过用[³H]二异丙基氟磷酸标记活性位点丝氨酸来鉴定活性位点肽段。肽段通过高效液相色谱法进行纯化。氨基酸组成和序列分析表明，来自常见基因型的肽段含有29个残基，序列为Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu-Leu-Ser-Pro-Gly-Ser-His-Ser-Leu-Phe-Thr-Arg。活性位点丝氨酸是N端的第八个残基。来自非典型基因型的含有活性位点丝氨酸的肽段含有22个残基，序列为Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu-Leu-Ser-Pro-Gly。来自非典型沉默基因型的肽段含有8个残基，序列为Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser。因此，非典型和非典型沉默活性位点肽段的序列与常见肽段的相应部分相同。
