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2
The purification and properties of the trimethoprim-resistant dihydrofolate reductase mediated by the R-factor, R388.由R因子R388介导的耐甲氧苄啶二氢叶酸还原酶的纯化及特性
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Purification and properties of dihydrofolate reductase from cultured mammalian cells.来自培养哺乳动物细胞的二氢叶酸还原酶的纯化及性质
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Site-directed mutagenesis of mouse dihydrofolate reductase. Mutants with increased resistance to methotrexate and trimethoprim.小鼠二氢叶酸还原酶的定点诱变。对甲氨蝶呤和甲氧苄啶耐药性增强的突变体。
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Trimethoprim-induced elevation of dihydrofolate reductase activity in human leukocytes.甲氧苄啶诱导人白细胞中二氢叶酸还原酶活性升高。
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引用本文的文献

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Antimicrobial susceptibility testing, drug resistance mechanisms, and therapy of infections with nontuberculous mycobacteria.非结核分枝杆菌感染的抗菌药物敏感性试验、耐药机制及治疗。
Clin Microbiol Rev. 2012 Jul;25(3):545-82. doi: 10.1128/CMR.05030-11.

本文引用的文献

1
Enzyme inhibition in relation to chemotherapy.与化疗相关的酶抑制作用。
Proc Soc Exp Biol Med. 1949 Oct;72(1):1-9. doi: 10.3181/00379727-72-17313.
2
Dihydrofolate reductase hysteresis and its effect of inhibitor binding analyses.二氢叶酸还原酶滞后现象及其抑制剂结合分析的作用
Biochemistry. 1981 Mar 31;20(7):1710-6. doi: 10.1021/bi00510a002.
3
Classification of trimethoprim-resistant dihydrofolate reductases mediated by R-plasmids using isoelectric focussing.使用等电聚焦法对由R质粒介导的耐甲氧苄啶二氢叶酸还原酶进行分类。
Eur J Biochem. 1982 Jul;125(3):617-22. doi: 10.1111/j.1432-1033.1982.tb06727.x.
4
Kinetics of methotrexate binding to dihydrofolate reductase from Neisseria gonorrhoeae.甲氨蝶呤与淋病奈瑟菌二氢叶酸还原酶结合的动力学
Biochem Pharmacol. 1984 Jul 1;33(13):2101-7. doi: 10.1016/0006-2952(84)90579-3.
5
R plasmid dihydrofolate reductase with a dimeric subunit structure.具有二聚体亚基结构的R质粒二氢叶酸还原酶。
J Biol Chem. 1983 Sep 25;258(18):10956-9.
6
Inhibition of folate biosynthesis and function as a basis for chemotherapy.抑制叶酸生物合成及以此作为化疗的基础。
Adv Enzymol Relat Areas Mol Biol. 1965;27:417-68. doi: 10.1002/9780470122723.ch9.
7
Inhibitor binding analysis of dihydrofolate reductases from various species.不同物种二氢叶酸还原酶的抑制剂结合分析
Mol Pharmacol. 1965 Sep;1(2):126-36.
8
Maturation of the head of bacteriophage T4. I. DNA packaging events.噬菌体T4头部的成熟。I. DNA包装事件。
J Mol Biol. 1973 Nov 15;80(4):575-99. doi: 10.1016/0022-2836(73)90198-8.
9
Trimethoprim resistance plasmids of different origin encode different drug-resistant dihydrofolate reductases.不同来源的甲氧苄啶耐药质粒编码不同的耐药二氢叶酸还原酶。
Plasmid. 1979 Jul;2(3):334-46. doi: 10.1016/0147-619x(79)90017-9.
10
Interaction of dihydrofolate reductase from amethopterin-resistant Lactobacillus casei with Cibacron Blue, Blue Dextran, and Affi-Gel Blue.来自氨甲蝶呤抗性干酪乳杆菌的二氢叶酸还原酶与汽巴克隆蓝、蓝色葡聚糖及亲和凝胶蓝的相互作用
J Biol Chem. 1979 Jul 25;254(14):6515-21.

草分枝杆菌二氢叶酸还原酶的纯化及特性研究

Purification and characterization of dihydrofolate reductase from Mycobacterium phlei.

作者信息

Al-Rubeai M, Dale J W

出版信息

Biochem J. 1986 Apr 1;235(1):301-3. doi: 10.1042/bj2350301.

DOI:10.1042/bj2350301
PMID:3741386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146682/
Abstract

The dihydrofolate reductase from Mycobacterium phlei was purified and characterized; it has an Mr of 15 000 and a pI of 4.8. It is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases.

摘要

纯化并鉴定了草分枝杆菌的二氢叶酸还原酶；其相对分子质量为15000，等电点为4.8。甲氨蝶呤和甲氧苄啶对其均有竞争性抑制作用，尽管其亲和力低于其他细菌的二氢叶酸还原酶。