MacManus J P, Watson D C, Yaguchi M
Biochem J. 1986 Apr 15;235(2):585-95. doi: 10.1042/bj2350585.
A 9000-Mr Ca2+-binding protein was isolated from rat placenta and purified to homogeneity by h.p.l.c. procedures. The complete amino acid sequence was established for the 78-residue placental protein. A sequence analysis of a minor component of the rat intestinal Ca2+-binding protein (residues 4-78) and a tryptic peptide (residues 55-74), both purified by h.p.l.c., showed both proteins to be identical. Thus this placental 9000-Mr Ca2+-binding protein is the same gene product as the intestinal Ca2+-binding protein whose synthesis is dependent on vitamin D.
从大鼠胎盘中分离出一种分子量为9000的钙离子结合蛋白，并通过高效液相色谱法将其纯化至同质。确定了这种由78个氨基酸残基组成的胎盘蛋白的完整氨基酸序列。对通过高效液相色谱法纯化的大鼠肠道钙离子结合蛋白的一个次要成分（第4至78位氨基酸残基）和一个胰蛋白酶肽段（第55至74位氨基酸残基）进行序列分析，结果表明这两种蛋白是相同的。因此，这种分子量为9000的胎盘钙离子结合蛋白与肠道钙离子结合蛋白是同一基因产物，后者的合成依赖于维生素D。
