Qian Zhengming, Huang Dingqiang, He Zhuobin, He Qinghui, Tan Guoying, Huang Qi, Sun Yikuo, Li Wenqing
College of Medical Imaging Laboratory and Rehabilitation, Xiangnan University, Chenzhou 423000, China.
Key Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., Dongguan 523850, Guangdong, China.
Int J Anal Chem. 2023 Jun 28;2023:5546053. doi: 10.1155/2023/5546053. eCollection 2023.
A rapid HPLC-UV method for the determination of three organic acids (neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid) in Polygoni Vivipari Rhizoma (PVR) by one marker was developed. The sample was prepared by effervescence-assisted matrix solid-phase dispersion (EA-MSPD). The separation of compounds was performed on a Poroshell column. The equal absorption wavelength was set as follows: 292 nm (0∼7 min) and 324 nm (7∼10 min). The analytical time including sample extraction and HPLC separation time was 12 min. The analytical method validation such as accuracy (recoveries 99.85%-106.29% and RSD < 2.9%), precision (RSD < 1.3%), reproducibility (RSD < 1.7%), and stability tests (RSD < 0.7% in 24 h) proved that the established HPLC method was suitable for determination of three organic acids in PVR. The contents of three analytes obtained by the external standard method with three markers and the equal absorption wavelength method with one marker were similar (RSD ≤ 2.0%). The developed method, which is rapid and reference compound saving, is an improved quality evaluation method of PVR.
建立了一种快速的HPLC-UV方法，通过单指标测定珠芽蓼根茎（PVR）中的三种有机酸（新绿原酸、绿原酸和隐绿原酸）。样品采用泡腾辅助基质固相分散（EA-MSPD）法制备。化合物的分离在Poroshell柱上进行。等吸收波长设置如下：292nm（0～7分钟）和324nm（7～10分钟）。包括样品提取和HPLC分离时间在内的分析时间为12分钟。分析方法验证，如准确度（回收率99.85%-106.29%，RSD＜2.9%）、精密度（RSD＜1.3%）、重现性（RSD＜1.7%）和稳定性试验（24小时内RSD＜0.7%）证明，所建立的HPLC方法适用于测定PVR中的三种有机酸。采用三指标外标法和单指标等吸收波长法获得的三种分析物含量相似（RSD≤2.0%）。所建立的方法快速且节省对照品，是一种改进后的PVR质量评价方法。
