Tan Daopeng, Yang Zhou, Zhang Qianru, Ling Hua, Du Yimei, Lu Yanliu, Xie Tianpei, Zhou Xumei, Qin Lin, He Yuqi
Key Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Guizhou 563009, China.
State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China.
Int J Anal Chem. 2020 Mar 18;2020:9731327. doi: 10.1155/2020/9731327. eCollection 2020.
A high-performance liquid chromatography method was developed for simultaneous quantification of 18 polyphenolic compounds from the leaves of , including 17 flavonoids and 1 phenylethanone. The extraction was separated by a Kromasil C column (250 × 4.6 mm, 5 m) with a binary gradient mobile phase consisting of acetonitrile and 0.2% aqueous acetic acid. A photodiode array detector (PDA) was used to record the signals of investigated constituents. The linearity, sensitivity, stability, precision, and accuracy of the established assay methods were assessed to meet the requirements of quantitative determination. Samples extracted by reflux in 25 mL of 80% methanol for 30 minutes were selected for the extraction method. The 18 compounds were accurately identified by comparing with the reference compounds. The purity of each peak was confirmed by the base peak in the mass spectrum. The contents of 18 compounds in Blumea samples from four different regions were successfully determined. The results also showed that 3,3',5,7-tetrahydroxy-4'-methoxyflavanone was the most abundant constituent, which could be used as a potential chemical marker for quality control of and Chinese patent medications containing herb.
建立了一种高效液相色谱法，用于同时定量测定[植物名称]叶片中的18种多酚类化合物，包括17种黄酮类化合物和1种苯乙酮。提取物通过Kromasil C柱（250×4.6 mm，5μm）分离，二元梯度流动相由乙腈和0.2%的乙酸水溶液组成。使用光电二极管阵列检测器（PDA）记录所研究成分的信号。对所建立的分析方法的线性、灵敏度、稳定性、精密度和准确度进行评估，以满足定量测定的要求。选择在25 mL 80%甲醇中回流提取30分钟的样品作为提取方法。通过与参考化合物比较，准确鉴定了这18种化合物。通过质谱中的基峰确认了每个峰的纯度。成功测定了来自四个不同地区的艾纳香样品中18种化合物的含量。结果还表明，3,3',5,7-四羟基-4'-甲氧基黄酮是含量最高的成分，可作为艾纳香及含艾纳香草药的中成药质量控制的潜在化学标志物。
