Rao K N, Kottapally S, Eskander E D, Shinozuka H, Dessi S, Pani P
Br J Cancer. 1986 Aug;54(2):305-10. doi: 10.1038/bjc.1986.177.
The regulation of cholesterol esterification during cell proliferation was studied. The serum free cholesterol, cholesterol esters and lecithin: cholesterol acyltransferase (LCAT) activity of nude mice with and without pancreatic acinar cell tumours and rats with proliferating tissues were determined. In addition, the apparent activity of acyl-CoA: cholesterol acyltransferase (ACAT) in homogenates of nude mouse tumours and proliferating rat tissues were determined and compared with those of normal nude mouse and rat tissues. Serum cholesterol ester levels were significantly lower in host nude mice with tumours and in rats with regenerating liver, and increased significantly in pregnant rats when compared with respective controls. Circulating LCAT activity levels decreased in host nude mice, in pregnant rats, and in rats with regenerating pancreas and regenerating liver. Apparent ACAT activity levels increased significantly in nude mouse tumours and in foetal and postnatal rat pancreata and also in postnatal liver. At the same time, apparent ACAT activity levels decreased in foetal and regenerating rat livers when compared with respective control tissues. These results suggest that serum cholesterol esters, circulating LCAT and cellular ACAT levels are modulated during cell proliferation.
对细胞增殖过程中胆固醇酯化的调节进行了研究。测定了有无胰腺腺泡细胞瘤的裸鼠以及具有增殖组织的大鼠的血清游离胆固醇、胆固醇酯和卵磷脂：胆固醇酰基转移酶（LCAT）活性。此外，还测定了裸鼠肿瘤匀浆和增殖大鼠组织中酰基辅酶A：胆固醇酰基转移酶（ACAT）的表观活性，并与正常裸鼠和大鼠组织的进行比较。与各自的对照组相比，患有肿瘤的宿主裸鼠和肝脏再生的大鼠血清胆固醇酯水平显著降低，而怀孕大鼠的血清胆固醇酯水平显著升高。宿主裸鼠、怀孕大鼠以及胰腺再生和肝脏再生的大鼠循环LCAT活性水平降低。裸鼠肿瘤、胎儿和出生后大鼠胰腺以及出生后肝脏中的ACAT表观活性水平显著升高。同时，与各自的对照组织相比，胎儿和再生大鼠肝脏中的ACAT表观活性水平降低。这些结果表明，在细胞增殖过程中，血清胆固醇酯、循环LCAT和细胞ACAT水平受到调节。
