Rothova A, van Knapen F, Baarsma G S, Kruit P J, Loewer-Sieger D H, Kijlstra A
Br J Ophthalmol. 1986 Aug;70(8):615-22. doi: 10.1136/bjo.70.8.615.
The diagnostic value of toxoplasma serology in ocular disease was evaluated in the following groups of patients: (I) uveitis cases of various causes (n = 291); (II) consecutive posterior and panuveitis patients (n = 60); (III) patients with definite congenital and ocular toxoplasmosis (n = 8); (IV) cases of clinical ocular toxoplasmosis (n = 25); and control patients with uveitis of non-toxoplasma origin (n = 12). No relation was observed between the level of the dye test titres and the diagnosis of ocular toxoplasmosis (groups I and II). During the active stages of the disease no typical change of the titres occurred in several longitudinally studied patients with toxoplasmosis. In group III one case was discovered to be negative by the dye test despite active ocular disease; however, IgG antibodies against toxoplasma were detected by the ELISA technique. In group IV, which was investigated by the ELISA technique, 100% of the toxoplasmosis patients were positive for IgG versus 58% of the control patients. Circulating immune complexes containing IgG and toxoplasma antigen were detected in seven of 25 toxoplasmosis patients (28%) and in two of 12 control patients (16%). Our study shows that the definite diagnosis of ocular toxoplasmosis or its exclusion by serological means only is not yet feasible. The possible superiority of the ELISA test to the dye test warrants further investigation.
（I）各种病因的葡萄膜炎病例（n = 291）；（II）连续性后葡萄膜炎和全葡萄膜炎患者（n = 60）；（III）确诊的先天性和眼部弓形虫病患者（n = 8）；（IV）临床眼部弓形虫病病例（n = 25）；以及非弓形虫源性葡萄膜炎的对照患者（n = 12）。未观察到染料试验滴度水平与眼部弓形虫病诊断之间的关联（I组和II组）。在疾病的活动期，几名纵向研究的弓形虫病患者的滴度未出现典型变化。在III组中，尽管有活动性眼部疾病，但有1例经染料试验检测为阴性；然而，通过ELISA技术检测到了针对弓形虫的IgG抗体。在通过ELISA技术进行研究的IV组中，100%的弓形虫病患者IgG呈阳性，而对照患者为58%。在25例弓形虫病患者中有7例（28%）和12例对照患者中有2例（16%）检测到含有IgG和弓形虫抗原的循环免疫复合物。我们的研究表明，仅通过血清学方法明确诊断眼部弓形虫病或排除该病尚不可行。ELISA试验相对于染料试验可能具有的优势值得进一步研究。
