Department of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, PR China.
Qlu School of Medicine, Shandong University, Jinan 502100, PR China.
J Inorg Biochem. 2023 Oct;247:112302. doi: 10.1016/j.jinorgbio.2023.112302. Epub 2023 Jun 22.
Isatin-3-(7'-Methoxychromone-3'-methylidene) hydrazone (L) was synthesized based on chromone schiff base, and used to construct a novel sensor to detect Cr. Fluorescence detection experiments were carried out for a range of different concentrations of Cr in aqueous solutions. A concentration calculation model was built on the basis of eliminating interference of excitation spectrum in the fluorescence spectra with mathematical method. Results showed that probe L displayed a 70-fold fluorescence enhancement upon the addition of Cr due to the photo-induced electron transfer (PET) effect. On the other hand, metal ions except Cr did not cause significant change in either the absorption or the fluorescence spectrum of L. In addition, L showed a good selectivity to Cr over other metal cations, especially Al and Cu. The probe L can detect Cr highly and selectively by the direct chelation enhanced fluorescence with a detection limit of 3.14 × 10 M. Furthermore, benefiting from their good water solubility and biocompatibility, cell imaging and real-time monitoring of Cr in living HepG2 cells were successfully achieved.
基于色酮席夫碱合成了色酮缩氨基腙（L），并将其用于构建一种新型传感器来检测 Cr。在水溶液中对不同浓度的 Cr 进行了荧光检测实验。通过数学方法消除荧光光谱中激发光谱的干扰，建立了浓度计算模型。结果表明，由于光诱导电子转移（PET）效应，探针 L 在加入 Cr 后荧光增强了 70 倍。另一方面，除 Cr 以外的金属离子不会导致 L 的吸收或荧光光谱发生显著变化。此外，L 对 Cr 具有良好的选择性，相对于其他金属阳离子，尤其是 Al 和 Cu，具有更高的选择性。探针 L 可以通过直接螯合增强荧光来高选择性地检测 Cr，检测限为 3.14×10−7 M。此外，由于其良好的水溶性和生物相容性，成功地实现了 Cr 在活 HepG2 细胞中的细胞成像和实时监测。
