School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, Foshan University, Foshan 528225, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Int J Biol Macromol. 2023 Aug 30;247:125716. doi: 10.1016/j.ijbiomac.2023.125716. Epub 2023 Jul 6.
In this study, a novel carboxymethylcellulose / ZnO / chitosan (CMC / ZnO / Cs) hydrogel microbeads loaded with crosslinked porous starch / curcumin (CPS / Cur) were designed and prepared to improve the encapsulation efficiency of curcumin for drug delivery to specific sites. It was found that the total pore volume of crosslinked porous starch (CPS) was increased by 1150 % when compared to the native starch (NS), and the adsorption ratio of curcumin by CPS was enhanced by 27 % when compared to NS. Secondly, the swelling ratio of composite hydrogel microbeads was within 25 % in an acidic environment at pH 1.2, and the swelling ratio of hydrogel microbeads sharply increased to 320 % ~ 370 % at pH 6.8 and 7.4. In addition, the results of in vitro simulated release experiments showed that the released amount of hydrogel microbeads loaded with NS/Cur and CPS/Cur in SGF were within 7 % in simulated gastric fluid (SGF). The highest released amount of curcumin was 65.26 % for hydrogel beads loaded with CPS/Cur, which was 26 % lower than that of hydrogel microbeads loaded with Cur in simulated intestinal fluid (SIF). In simulated colonic fluid (SCF), the released amount of hydrogel microbeads loaded with CPS/Cur and Cur were 73.96 % and 91.69 %, respectively. In conclusion, pH-sensitive drug delivery system with good drug stability and bioavailability were successfully prepared with carboxymethylcellulose / ZnO / chitosan bead, suitable targeting drug delivery to the small intestine.
在这项研究中，设计并制备了一种新型的羧甲基纤维素/氧化锌/壳聚糖（CMC/ZnO/Cs）水凝胶微球，负载交联多孔淀粉/姜黄素（CPS/姜黄素），以提高姜黄素的包封效率，实现药物的靶向传递。研究发现，与天然淀粉（NS）相比，交联多孔淀粉（CPS）的总孔体积增加了 1150%，CPS 对姜黄素的吸附率提高了 27%。其次，复合水凝胶微球在酸性环境（pH 1.2）中的溶胀率在 25%以内，而在 pH 6.8 和 7.4 时，水凝胶微球的溶胀率急剧增加到 320%~370%。此外，体外模拟释放实验结果表明，载有 NS/Cur 和 CPS/Cur 的水凝胶微球在 SGF 中的释放量在模拟胃液（SGF）中均低于 7%。载有 CPS/Cur 的水凝胶微球中姜黄素的最大释放量为 65.26%，而在模拟肠液（SIF）中载有 Cur 的水凝胶微球的释放量为 81.26%，载有 CPS/Cur 的水凝胶微球的释放量低 26%。在模拟结肠液（SCF）中，载有 CPS/Cur 和 Cur 的水凝胶微球的释放量分别为 73.96%和 91.69%。综上所述，成功制备了具有良好药物稳定性和生物利用度的 pH 敏感型载药系统，适用于小肠的靶向药物传递。
