Polymer Research Laboratory, Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran.
Polymer Research Laboratory, Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, P.O. Box 51666, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Science, Tabriz, Iran.
Colloids Surf B Biointerfaces. 2023 Nov;231:113585. doi: 10.1016/j.colsurfb.2023.113585. Epub 2023 Oct 10.
In this study, a pH-responsive hydrogels based on laponite rapid dispersion (Lap®)/chitosan (CS)/polyvinyl alcohol (PVA) designed and was used for controlled delivery of the anticancer drug curcumin (CUR). First, it was accomplished by dissolving CUR in Lap® dispersion under the influence of the pH of the environment. Then, in the presence of Lap®CUR cross-linking was incorporated between CS and PVA polymers. The structural features of Lap®CUR/CS@PVA hydrogels are characterized using FT-IR, XRD, SEM/EDS, TEM, TGA, Zeta potential, and XPS. The in vitro drug release profiles confirmed a pH-responsive controlled release of CUR in acidic pH for all hydrogels. During 12 h, the cumulative release of CUR from Lap®CUR/0.1CS@PVA hydrogel was 27.9% and 12.3%, at pH 5.5 and 7.4, respectively. While during three days the release rate reached 48.5% and 18.5%. The CUR release kinetic from hydrogels also suggests that the kinetic data well fitted to the Korsmeyer-Peppas, diffusion-controlled and Fickian diffusion. Furthermore, in vitro cytotoxicity and DAPI staining study clearly illustrated that Lap®CUR/0.1CS@PVA hydrogel had lower cytotoxicity than CUR against MDA-MB 231 cancer cells, which confirmed the controlled release of drug through hydrogels. Meanwhile, in vitro hemolysis, antioxidant and antibacterial tests revealed that the prepared hydrogels have good blood compatibility, excellent antioxidant properties, and antibacterial activity. Based on the obtained results, the designed hydrogels could be potentially applied as pH-controlled drug delivery systems for cancer therapy.
在这项研究中，设计了一种基于快分散蒙脱土（Lap®）/壳聚糖（CS）/聚乙烯醇（PVA）的 pH 响应水凝胶，用于控制抗癌药物姜黄素（CUR）的递送。首先，通过在环境 pH 的影响下将 CUR 溶解在 Lap®分散体中来完成。然后，在 Lap®CUR 的存在下，CS 和 PVA 聚合物之间发生了交联。使用 FT-IR、XRD、SEM/EDS、TEM、TGA、Zeta 电位和 XPS 对 Lap®CUR/CS@PVA 水凝胶的结构特征进行了表征。体外药物释放曲线证实了所有水凝胶在酸性 pH 下均能实现 CUR 的 pH 响应控制释放。在 12 小时内，Lap®CUR/0.1CS@PVA 水凝胶在 pH 5.5 和 7.4 下的 CUR 累积释放率分别为 27.9%和 12.3%。而在三天内，释放速率达到 48.5%和 18.5%。水凝胶中 CUR 的释放动力学也表明，动力学数据很好地符合 Korsmeyer-Peppas、扩散控制和菲克扩散。此外，体外细胞毒性和 DAPI 染色研究清楚地表明，Lap®CUR/0.1CS@PVA 水凝胶对 MDA-MB 231 癌细胞的细胞毒性低于 CUR，这证实了通过水凝胶控制药物释放。同时，体外溶血、抗氧化和抗菌试验表明，所制备的水凝胶具有良好的血液相容性、优异的抗氧化性能和抗菌活性。基于获得的结果，设计的水凝胶可作为用于癌症治疗的 pH 控制药物递送系统。
