Mazur M, Younglai E V
Biol Reprod. 1986 Aug;35(1):191-7. doi: 10.1095/biolreprod35.1.191.
The involvement of the pituitary and mesonephros in oogenesis was examined with the rabbit used as an experimental model. Fetal and neonatal ovaries were cultured for 8 days with and without their corresponding pituitaries or as half cranial or caudal portions. Progression of meiosis was followed histologically and autoradiographically by the uptake of tritiated thymidine. Pulse labeling for 24 h occurred on Days 0, 2, 4 and 7 of culture. No difference was noted in labeling or progression of meiosis in neonatal ovaries with or without the pituitary or in sectioned ovaries. By contrast, fetal ovaries with pituitary that were labeled on Day 4 had a labeling index (positive cells/1000 microns 2 of cortex) of 282.1 +/- 32.9 (mean +/- SEM) compared to 107.1 +/- 24.9 for ovaries without pituitary. Those labeled on Day 7 showed the same differences (263.7 +/- 46.0 vs. 160.2 +/- 20.8). Histologically, there was a threefold difference in percentage of germ cells with pituitary undergoing meiosis (21.2 +/- 2.9% compared to 7.1 +/- 2.8% without). These data suggest that the fetal pituitary has an important role in inducing meiotic prophase in the rabbit.
以兔作为实验模型，研究垂体和中肾在卵子发生中的作用。将胎儿和新生兔的卵巢分别与相应的垂体一起培养或不与垂体一起培养，或者切成半脑侧或尾侧部分培养8天。通过组织学方法以及利用氚标记胸腺嘧啶核苷的摄取进行放射自显影来追踪减数分裂的进程。在培养的第0、2、4和7天进行24小时脉冲标记。在有无垂体的新生兔卵巢中，或者在切片卵巢中，标记或减数分裂进程均未观察到差异。相比之下，在第4天标记的带有垂体的胎儿卵巢，其标记指数（每1000平方微米皮质中的阳性细胞数）为282.1±32.9（平均值±标准误），而没有垂体的卵巢为107.1±24.9。在第7天标记的卵巢也显示出同样的差异（263.7±46.0对160.2±20.8）。组织学检查显示，带有垂体的生殖细胞进行减数分裂的百分比存在三倍差异（21.2±2.9%，而没有垂体的为7.1±2.8%）。这些数据表明，胎儿垂体在诱导兔减数分裂前期方面具有重要作用。
