Gapienko E F, Markelova I V
Biull Eksp Biol Med. 1976 Jun;81(6):735-8.
The authors compared the extent of development of the germ cells of the human fetus ovaries developing in the organ culture and of control ovaries obtained of fetuses of "equivalent" age. The capacity to enter the meiosis prophase under conditions of the organ culture was expressed by the germ cells of fetuses of 8-9 week of gestation, and older; only individual oocytes reached the leptotene stage in the explants of the ovaries of 7-8 week fetuses. The oocytes at the leptotene, zygotene and pachitene stage appeared in the culture at the same periods as in the organism. However, the percentage of cells of these stages in the explant was as a rule lower than in control ovaries. In case of pulse administration of thymidine-3H into the culture labeled oocytes at the zygotene stage appeared in 4, and at the pachitene stage - in 14 days.
作者比较了在器官培养中发育的人类胎儿卵巢生殖细胞的发育程度，以及取自“同等”年龄胎儿的对照卵巢的生殖细胞发育程度。妊娠8 - 9周及更大孕周胎儿的生殖细胞表现出在器官培养条件下进入减数分裂前期的能力；7 - 8周胎儿卵巢外植体中只有个别卵母细胞达到细线期。细线期、偶线期和粗线期的卵母细胞在培养物中出现的时期与在机体中相同。然而，外植体中这些阶段细胞的百分比通常低于对照卵巢。当向培养物中脉冲注入3H - 胸腺嘧啶核苷时，偶线期标记的卵母细胞在4天后出现，粗线期的在14天后出现。
