State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, 071001, Hebei, China.
Theor Appl Genet. 2023 Jul 7;136(8):171. doi: 10.1007/s00122-023-04415-w.
A. gossypii resistance showed great variability in G. hirsutum varieties. One hundred and seventy-six SNPs associated with A. gossypii resistance were identified using GWAS. Four candidate resistance genes were functionally validated. Aphis gossypii is an economically important sap-feeding pest and is widely distributed in the world's cotton-producing regions. Identification of cotton genotypes and developing cultivars with improved A. gossypii resistance (AGR) is essential and desirable for sustainable agriculture. In the present study, A. gossypii was offered no choice but to propagate on 200 Gossypium hirsutum accessions. A relative aphid reproduction index (RARI) was used to evaluate the AGR, which showed large variability in cotton accessions and was classified into 6 grades. A significantly positive correlation was found between AGR and Verticillium wilt resistance. A total of 176 SNPs significantly associated with the RARI were identified using GWAS. Of these, 21 SNPs could be repeatedly detected in three replicates. Cleaved amplified polymorphic sequence, a restriction digestion-based genotyping assay, was developed using SNP1 with the highest observed -log(P-value). Four genes within the 650 kb region of SNP1 were further identified, including GhRem (remorin-like), GhLAF1 (long after far-red light 1), GhCFIm25 (pre-mRNA cleavage factor Im 25 kDa subunit) and GhPMEI (plant invertase/pectin methylesterase inhibitor superfamily protein). The aphid infection could induce their expression and showed a significant difference between resistant and susceptible cotton varieties. Silencing of GhRem, GhLAF1 or GhCFIm25 could significantly increase aphid reproduction on cotton seedlings. Silencing of GhRem significantly reduced callose deposition, which is reasonably believed to be the cause for the higher AGR. Our results provide insights into understanding the genetic regulation of AGR in cotton and suggest candidate germplasms, SNPs and genes for developing cultivars with improved AGR.
A. 棉蚜对陆地棉品种的抗性表现出很大的变异性。通过 GWAS 鉴定出与 A. 棉蚜抗性相关的 176 个 SNP。对 4 个候选抗性基因进行了功能验证。棉蚜是一种重要的经济上的刺吸式害虫，广泛分布于世界棉花产区。鉴定棉花基因型和培育具有改良 A. 棉蚜抗性（AGR）的品种对于可持续农业是必不可少和理想的。在本研究中，A. 棉蚜只能在 200 个陆地棉品种上繁殖。相对蚜虫繁殖指数（RARI）用于评估 AGR，发现棉花品种间的 AGR 存在很大的变异性，并分为 6 个等级。AGR 与黄萎病抗性呈显著正相关。通过 GWAS 共鉴定出与 RARI 显著相关的 176 个 SNP，其中 21 个 SNP 在 3 次重复中均可重复检测到。利用 SNP1 开发了基于 SNP1 切割扩增多态性序列（cleaved amplified polymorphic sequence，CAPS）的限制性内切酶酶切基因分型检测方法，观察到的 SNP1 的最高-log(P 值)为-10.64。在 SNP1 650 kb 区域内进一步鉴定出 4 个基因，包括 GhRem（remorin-like）、GhLAF1（long after far-red light 1）、GhCFIm25（pre-mRNA cleavage factor Im 25 kDa subunit）和 GhPMEI（plant invertase/pectin methylesterase inhibitor superfamily protein）。蚜虫感染可诱导其表达，并在抗性和感病棉花品种之间表现出显著差异。沉默 GhRem、GhLAF1 或 GhCFIm25 可显著增加棉苗上蚜虫的繁殖。沉默 GhRem 可显著减少胼胝质的沉积，这被认为是 AGR 较高的原因。我们的研究结果为深入了解棉花 AGR 的遗传调控机制提供了思路，并为培育具有改良 AGR 的品种提供了候选种质资源、SNP 和基因。
