用于液滴微流控中磁性珠均匀分散的摇动装置。

Shaking Device for Homogeneous Dispersion of Magnetic Beads in Droplet Microfluidics.

机构信息

Department of Physics and Astronomy, University of Padua, Via Marzolo 8, 35131 Padua, Italy.

出版信息

Sensors (Basel). 2023 Jun 7;23(12):5399. doi: 10.3390/s23125399.

Abstract

Magnetic beads (or particles) having a size between 1 and 5 µm are largely used in many biochemical assays devoted to both purification and quantification of cells, nucleic acids, or proteins. Unfortunately, the use of these beads within microfluidic devices suffers from natural precipitation because of their size and density. The strategies applied thus far to cells or polymeric particles cannot be extended to magnetic beads, mainly due to their magnetization and their higher densities. We report an effective shaking device capable of preventing the sedimentation of beads that are stored in a custom PCR tube. After the characterization of the operating principle, the device is validated for magnetic beads in droplets, leading to an equal distribution between the droplets, barely affecting their generation.

摘要

粒径在 1 至 5 微米之间的磁珠在许多用于细胞、核酸或蛋白质的纯化和定量的生化分析中得到了广泛的应用。不幸的是,由于其尺寸和密度,这些磁珠在微流控装置中的使用会出现自然沉淀的问题。迄今为止,应用于细胞或聚合物颗粒的策略不能扩展到磁性珠,主要是由于它们的磁化和更高的密度。我们报告了一种有效的摇床装置,能够防止储存在定制 PCR 管中的珠子沉淀。在对工作原理进行了表征之后,该装置在液滴中的磁珠得到了验证,导致液滴之间的分布均匀,几乎不会影响液滴的生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4efc/10304097/520a552ab5c1/sensors-23-05399-g001.jpg

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