Park Juhwan, Lee Kyoung G, Han Dong Hyun, Lee Ji-Soo, Lee Seok Jae, Park Je-Kyun
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Nanobio Application Team, National Nanofab Center (NNFC), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Biosens Bioelectron. 2021 Jun 1;181:113159. doi: 10.1016/j.bios.2021.113159. Epub 2021 Mar 13.
Here, we report a portable microfluidic device to generate and dispense droplets simply operated by pushbutton for droplet digital polymerase chain reaction (ddPCR), which is named pushbutton-activated microfluidic dropenser (droplet dispenser) (PAMD). After loading the PCR mixtures and the droplet generation oil to PAMD, digitized PCR mixtures are prepared in PCR tubes after the actuation of a pushbutton. Multiple droplet generation units are simultaneously operated by a single pushbutton, and the size of droplets is controllable by adjusting the geometry of the droplet generation channel. To examine the performance of PAMD, digitized PCR mixtures containing genomic DNA of Escherichia coli (E. coli) O157:H7 prepared by PAMD were assessed by a fluorescence signal analyzer after PCR with a thermal cycler. As a result, PAMD can produce analytical droplets for ddPCR as much as a conventional droplet generator even though any external equipment is not required.
在此，我们报告一种便携式微流控装置，用于通过按钮简单操作来生成和分配液滴，用于数字液滴聚合酶链反应（ddPCR），该装置名为按钮激活微流控液滴分配器（PAMD）。将PCR混合物和液滴生成油加载到PAMD中后，按下按钮即可在PCR管中制备数字化的PCR混合物。多个液滴生成单元可通过单个按钮同时操作，并且通过调整液滴生成通道的几何形状可控制液滴大小。为了检测PAMD的性能，在使用热循环仪进行PCR后，通过荧光信号分析仪对由PAMD制备的含有大肠杆菌O157:H7基因组DNA的数字化PCR混合物进行评估。结果表明，即使不需要任何外部设备，PAMD也能产生与传统液滴发生器一样多的用于ddPCR的分析液滴。
