College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, PR China.
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, PR China.
Gene. 2023 Sep 5;880:147624. doi: 10.1016/j.gene.2023.147624. Epub 2023 Jul 6.
Enhancing meat production and quality is the eternal theme for pig breeding industries. Fat deposition has always been the focus of research in practical production because it is closely linked to pig production efficiency and pork quality. In the current study, multi-omics techniques were performed to explore the modulatory mechanisms of backfat (BF) accumulation at three core developmental stages for Ningxiang pigs. Our results identified that 15 differentially expressed genes (DEGs) and 9 significantly changed metabolites (SCMs) contributed to the BF development via the cAMP signaling pathway, regulation of lipolysis in adipocytes, and biosynthesis of unsaturated fatty acids. Herein, we found a series of candidate genes such as adrenoceptor beta 1 (ADRB1), adenylate cyclase 5 (ADCY5), ATPase Na+/K+ transporting subunit beta 1 (ATP1B1), ATPase plasma membrane Ca transporting 3 (ATP2B3), ATPase Na+/K+ transporting subunit alpha 2 (ATP1A2), perilipin 1 (PLIN1), patatin like phospholipase domain containing 3 (PNPLA3), ELOVL fatty acid elongase 5 (ELOVL5) and metabolites like epinephrine, cAMP, arachidonic acid, oleic acid, linoleic acid, and docosahexaenoic acid existed age-specificeffects and played important roles in lipolysis, fat accumulation, and fatty acid composition. Our findings provide a reference for molecular mechanisms in BF tissue development and the optimization of carcass quality.
提高肉产量和品质是养猪业永恒的主题。脂肪沉积一直是实际生产研究的重点，因为它与猪生产效率和猪肉质量密切相关。在本研究中，采用多组学技术研究了宁乡猪三个核心发育阶段背部脂肪（BF）积累的调控机制。我们的研究结果表明，15 个差异表达基因（DEGs）和 9 个显著变化的代谢物（SCMs）通过 cAMP 信号通路、脂肪细胞脂解调节和不饱和脂肪酸的生物合成，对 BF 发育有贡献。在此，我们发现了一系列候选基因，如肾上腺素能受体β 1（ADRB1）、腺苷酸环化酶 5（ADCY5）、Na+/K+ 转运ATP 酶β 1 亚基（ATP1B1）、ATP 酶质膜 Ca 转运 3（ATP2B3）、Na+/K+ 转运ATP 酶α 2 亚基（ATP1A2）、脂滴包被蛋白 1（PLIN1）、patatin 样磷脂酶结构域包含 3（PNPLA3）、ELOVL 脂肪酸延长酶 5（ELOVL5）和代谢物，如肾上腺素、cAMP、花生四烯酸、油酸、亚油酸和二十二碳六烯酸，具有年龄特异性效应，在脂肪分解、脂肪积累和脂肪酸组成中发挥重要作用。我们的研究结果为 BF 组织发育的分子机制和胴体品质的优化提供了参考。
