College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350116, China; MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350116, China; MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China.
Anal Chim Acta. 2023 Sep 8;1273:341544. doi: 10.1016/j.aca.2023.341544. Epub 2023 Jun 19.
Point-of-care testing (POCT) has experienced rapid development owing to its advantages of rapid testing, low cost and strong operability, making it indispensable for analyte detection in outdoor or rural areas. In this study, we propose a novel method for the detection of aflatoxin B (AFB) using a dual-signal readout approach within a unified system. This method employs dual channel modes, namely visual fluorescence and weight measurements, as the signal readouts. Specifically, a pressure-sensitive material is utilized as a visual fluorescent agent, its signal can be quenched in the presence of high oxygen pressure. Additionally, an electronic balance, commonly used for weight measurement, is adopted as another signal device, where the signal is generated through the catalytic decomposition of HO by platinum nanoparticles. The experimental results demonstrate that the proposed device enables accurate AFB detection within the concentration range of 1.5-32 μg mL, with a detection limit of 0.47 μg mL. Moreover, this method has been successfully applied for practical AFB detection with satisfactory results. Notably, this study pioneers the use of a pressure-sensitive material as a visual signal in POCT. By addressing the limitations of single-signal readout approaches, our method fulfills requirements of intuitiveness, sensitivity, quantitative analysis and reusability.
即时检测（POCT）因其检测快速、成本低、操作性强等优点而得到迅猛发展，成为户外或农村地区进行分析物检测必不可少的手段。本研究提出了一种在统一系统中使用双信号读出方法检测黄曲霉毒素 B（AFB）的新方法。该方法采用双通道模式，即视觉荧光和重量测量作为信号读出。具体来说，压力敏感材料被用作视觉荧光剂，其信号在高氧压力存在下会被猝灭。此外，电子天平（通常用于称重）被用作另一种信号装置，其中信号通过铂纳米颗粒对 HO 的催化分解产生。实验结果表明，该装置能够在 1.5-32μgmL 的浓度范围内实现准确的 AFB 检测，检测限为 0.47μgmL。此外，该方法已成功应用于实际的 AFB 检测，取得了令人满意的结果。值得注意的是，本研究首次将压力敏感材料用作 POCT 的视觉信号。通过解决单信号读出方法的局限性，我们的方法满足了直观性、灵敏度、定量分析和可重复使用性的要求。
