Wu Jizhong, Jia Haiyan, Qiao Linyi, Fu Bisheng, Brown-Guedira Gina, Nagarajan Ragupathi, Yan Liuling
Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014 Jiangsu China.
Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078 USA.
Mol Breed. 2023 Jul 6;43(7):56. doi: 10.1007/s11032-023-01402-3. eCollection 2023 Jul.
European winter wheat cultivar "Tabasco" was reported to have resistance to powdery mildew disease caused by f. sp. () in China. In previous studies, Tabasco was reported to have the resistance gene designated as on the short arm of chromosome 5D when a mapping population was phenotyped with pathogen isolate collected in China and was genotyped with simple sequence repeat (SSR) markers. In this study, single-nucleotide polymorphism (SNP) chips were used to rapidly determine the resistance gene by mapping a new F population that was developed from Tabasco and a susceptible cultivar "Ningmaizi119" and inoculated with pathogen isolate NCF-D-1-1 that was collected in the USA. The segregation of resistance in the population was found to link with which was identified in Tabasco. Therefore, it was concluded that the previously reported on chromosome arm 5DS in Tabasco should be the gene on the same chromosome. The was also found in European cultivars "Mattis" and "Claire" but not in any of the accessions from diploid wheat or modern cultivars such as "Gallagher," "Smith's Gold," and "OK Corral" being used in the Great Plains in the USA. A KASP marker was developed to track the resistance allele in wheat breeding.
The online version contains supplementary material available at 10.1007/s11032-023-01402-3.
据报道，欧洲冬小麦品种“塔巴斯科”对中国的小麦白粉菌引起的白粉病具有抗性。在先前的研究中，当用在中国收集的病原菌分离物对一个作图群体进行表型分析，并使用简单序列重复（SSR）标记进行基因分型时，报道称塔巴斯科在5D染色体短臂上具有名为 的抗性基因。在本研究中，通过对由塔巴斯科和感病品种“宁麦籽119”培育出的一个新的F群体进行作图，并接种在美国收集的病原菌分离物NCF-D-1-1，使用单核苷酸多态性（SNP）芯片快速确定抗性基因。发现该群体中的抗性分离与在塔巴斯科中鉴定出的 相关。因此，得出结论，先前报道的塔巴斯科5DS染色体臂上的 应该是同一条染色体上的 基因。在欧洲品种“马蒂斯”和“克莱尔”中也发现了 ，但在美国大平原使用的二倍体小麦的任何种质或现代品种如“加拉格尔”、“史密斯黄金”和“OK科拉尔”中均未发现。开发了一种竞争性等位基因特异性PCR（KASP）标记来追踪小麦育种中的抗性等位基因 。
在线版本包含可在10.1007/s11032-023-01402-3获取的补充材料。
