Lactate based caproate production with and process control of via lactate dependent electrolysis.

Syngas fermentation processes with acetogens represent a promising process for the reduction of CO emissions alongside bulk chemical production. However, to fully realize this potential the thermodynamic limits of acetogens need to be considered when designing a fermentation process. An adjustable supply of H as electron donor plays a key role in autotrophic product formation. In this study an anaerobic laboratory scale continuously stirred tank reactor was equipped with an All-in-One electrode allowing for H generation via electrolysis. Furthermore, this system was coupled to online lactate measurements to control the co-culture of a recombinant lactate-producing strain and a lactate-consuming strain to produce caproate. When was grown in batch cultivations with lactate as substrate, 1.6 g·L caproate were produced. Furthermore, lactate production of the mutant strain could manually be stopped and reinitiated by controlling the electrolysis. Applying this automated process control, lactate production of the mutant strain could be halted to achieve a steady lactate concentration. In a co-culture experiment with the mutant strain and the strain, the automated process control was able to dynamically react to changing lactate concentrations and adjust H formation respectively. This study confirms the potential of as medium chain fatty acid producer in a lactate-mediated, autotrophic co-cultivation with an engineered strain. Moreover, the monitoring and control strategy presented in this study reinforces the case for autotrophically produced lactate as a transfer metabolite in defined co-cultivations for value-added chemical production.