Gupta Seema, Sharma Manish, Banerjee Satyabrat, Holikatti Kiran, Kamble Priyanka, Goyal Jay V
Orthodontics, Jawahar Medical Foundation's (JMF) Annasaheb Chudaman Patil Memorial (ACPM) Dental College, Dhule, IND.
Oral Pathology and Microbiology, Jawahar Medical Foundation's (JMF) Annasaheb Chudaman Patil Memorial (ACPM) Dental College, Dhule, IND.
Cureus. 2023 Jun 6;15(6):e40069. doi: 10.7759/cureus.40069. eCollection 2023 Jun.
Background Oral submucous fibrosis (OSMF) is a prevalent precancerous condition of the oral cavity and an ambiguity to clinicians because of its indistinguishable etiopathogenesis. Previous studies could not establish a definite role of mast cells (MCs) in the fibrosis of stroma. The present study was done to study the histopathological changes in OSMF and to determine the association of mast cells (MCs) and their degranulated components with vascularity. Methods A retrospective case-control study involved 40 cases of various histopathological grades of OSMF and was compared with 10 cases of normal buccal mucosa by using a cluster of differentiation 117(CD117) kit for the identification of MCs and Masson's trichrome stain to study the number of blood vessels (BVs). Results The present study indicated that advanced cases of OSMF had keratinized epithelium with atrophic changes and moderate to advanced fibrosis of stroma with the involvement of underlying muscles. The MC density and the number of blood vessels were progressively reduced in OSMF as the grade advanced compared to healthy controls. Conclusion An increase in the mast cell density in the initial stages of OSMF suggests their definite role in the initiation of fibrosis and secondary changes to the epithelium such as atrophy.
口腔黏膜下纤维化（OSMF）是一种常见的口腔癌前病变，因其发病机制不明，给临床医生带来了困扰。以往的研究未能确定肥大细胞（MCs）在基质纤维化中的明确作用。本研究旨在研究OSMF的组织病理学变化，并确定肥大细胞（MCs）及其脱颗粒成分与血管生成的关系。方法：一项回顾性病例对照研究纳入了40例不同组织病理学分级的OSMF病例，并与10例正常颊黏膜病例进行比较，使用分化簇117（CD117）试剂盒鉴定MCs，并用Masson三色染色法研究血管数量（BVs）。结果：本研究表明，OSMF晚期病例有角化上皮萎缩改变，基质中度至重度纤维化，并累及深层肌肉。与健康对照组相比，随着OSMF分级的进展，MC密度和血管数量逐渐减少。结论：OSMF早期肥大细胞密度增加表明它们在纤维化起始及上皮萎缩等继发性改变中起明确作用。
