Salmon R J, Buisson J P, Zafrani B, Aussepe L, Royer R
Carcinogenesis. 1986 Sep;7(9):1447-50. doi: 10.1093/carcin/7.9.1447.
7-methoxy-2-nitro-naphtho[2,1-b] furan (R 7000), known as a very potent mutagen and a very active in vivo carcinogen was employed here to develop squamous cell carcinoma in the rat. Seventy male Wistar rats received R 7000 p.o. dissolved in deionized water with 5% ethanol for periods from 1 to 21 months, while 20 served as controls receiving either water or 5% ethanol. All the animals were killed 21 months after the beginning of the experiment. Microscopic lesions were noticed in the forestomach after only 1 month of R 7000 administration. Histologic features varied from slight dysplasia to invasive carcinoma. Their sizes and invasive character increased significantly with the amount of R 7000 administered (P less than 0.05). R 7000 can be considered as a locally acting carcinogen since its carcinogenic effects appear at a place where the compound collects after swallowing. R 7000 is a weak carcinogen at the concentration employed here, when compared to some nitrosamines used in the development of forestomach cancer in rodents. The exact mechanism of the carcinogenic effects remains to be explained.
7-甲氧基-2-硝基萘并[2,1-b]呋喃（R 7000），一种已知的强效诱变剂和活性很强的体内致癌物，在此被用于诱导大鼠发生鳞状细胞癌。70只雄性Wistar大鼠经口给予溶解于含5%乙醇的去离子水中的R 7000，给药1至21个月，而20只作为对照组，给予水或5%乙醇。在实验开始21个月后处死所有动物。在给予R 7000仅1个月后，在前胃就观察到微观病变。组织学特征从轻度发育异常到浸润性癌不等。它们的大小和浸润特征随R 7000给药量的增加而显著增加（P小于0.05）。R 7000可被视为局部作用的致癌物，因为其致癌作用出现在该化合物吞咽后聚集的部位。与用于诱导啮齿动物前胃癌的一些亚硝胺相比，在此所用浓度下R 7000是一种弱致癌物。致癌作用的确切机制仍有待阐明。
