Department of Biology Kutztown University of Pennsylvania Kutztown, Pennsylvania19530USA.
Rodale Institute Kutztown, Pennsylvania19530USA.
J Helminthol. 2023 Jul 10;97:e55. doi: 10.1017/S0022149X23000391.
Nodular roundworms ( spp.) are frequent parasites of the large intestine in several mammal species including humans and pigs, and their study often requires the use of infective larvae produced using several coproculture techniques. However, there is no published comparison of techniques to determine which yields the highest number of larvae. This study compares the number of larvae recovered from coprocultures made with charcoal, sawdust, vermiculite, and water in an experiment repeated twice using feces from a sow naturally infected with spp. at an organic farm. A higher number of larvae were recovered from coprocultures using sawdust relative to other types of media used, and this was consistent across the two trials. The use of sawdust to culture spp. larvae is rarely reported and our study suggests it can yield higher numbers relative to other media.
结节蛔虫（ spp.）是包括人类和猪在内的几种哺乳动物大肠内的常见寄生虫，其研究通常需要使用几种粪便培养技术来产生感染性幼虫。然而，目前还没有发表过比较这些技术的文章，以确定哪种技术能产生最多的幼虫。本研究使用来自有机农场中自然感染 spp.的母猪粪便，在两次重复的实验中，比较了用木炭、锯末、蛭石和水制作的粪便培养物中回收的幼虫数量。与使用的其他类型的培养基相比，锯末中回收的幼虫数量更多，这在两次试验中都是一致的。很少有报道使用锯末来培养 spp.幼虫，我们的研究表明，与其他培养基相比，它可以产生更多的幼虫。
