State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, P. R China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Food Funct. 2023 Jul 31;14(15):6896-6913. doi: 10.1039/d3fo00625e.
Clinical antibiotics used worldwide could diminish the intestinal barrier, enhance contact with microbiota and intestinal immune cells, and induce inflammation. We found that ciprofloxacin treatment of serovar Typhimurium infection resulted in the destruction of the intestinal barrier, with decreased concentrations of MUC2, ZO-1, and occludin in the jejunum and colon. ethanol extracts (GLE), as a prebiotic food extract, significantly decreased inflammation-related enzymes, including COX-2, MPO, and iNOS, and pro-inflammatory cytokines (IL-6, IL-1β, IL-17, and TNF-α), and protected the intestinal barrier by increasing the concentration of MUC2, ZO-1, and occludin. Meanwhile it significantly increased the abundances of , , , , and , which increased the risk of pathogenic bacterial infections. Prebiotic polysaccharide (GLP) provided a significant intestinal barrier, improving the concentration of ZO-1, occludin, and MUC2 in the colon and jejunum. The synergistic effects of GLP and ciprofloxacin were hypothesized to reverse the negative effects resulting from ciprofloxacin alone, as the concentrations of ZO-1, occludin, and MUC2 were significantly increased in the jejunum and colon, especially in the colon. Also, the synergistic effect increased the abundances of probiotic bacteria NK4A136, UGG-014, , and . In conclusion, combined GLP and ciprofloxacin therapy against infection alleviated the side effects resulting from the clinical application of the antibiotic alone, and increased the probiotic bacterial population.
全球临床使用的抗生素会破坏肠道屏障，增加与微生物群和肠道免疫细胞的接触，并引发炎症。我们发现环丙沙星治疗肠炎沙门氏菌感染会导致肠道屏障破坏，空肠和结肠中 MUC2、ZO-1 和闭合蛋白的浓度降低。乙醇提取物（GLE）作为一种益生元食品提取物，可显著降低炎症相关酶，包括 COX-2、MPO 和 iNOS，以及促炎细胞因子（IL-6、IL-1β、IL-17 和 TNF-α），并通过增加 MUC2、ZO-1 和 occludin 的浓度来保护肠道屏障。同时，它还显著增加了 、 、 、 、和 ，这些菌增加了致病性细菌感染的风险。益生元多糖（GLP）提供了显著的肠道屏障，增加了结肠和空肠中 ZO-1、occludin 和 MUC2 的浓度。GLP 和环丙沙星的协同作用假设可以逆转环丙沙星单独使用产生的负面影响，因为空肠和结肠中 ZO-1、occludin 和 MUC2 的浓度显著增加，尤其是在结肠中。此外，协同作用增加了益生菌细菌 NK4A136、UGG-014、 和 的丰度。总之，GLP 和环丙沙星联合治疗肠炎沙门氏菌感染减轻了抗生素单独临床应用产生的副作用，并增加了益生菌细菌的数量。
