Tharapel A T, Dacus J V, Tharapel S A, Dempsey J, Flinn G, Shaver D C, Massouda D, Wilroy R S
Clin Genet. 1986 Jun;29(6):502-7. doi: 10.1111/j.1399-0004.1986.tb00551.x.
Chorionic villi sampling was performed on 52 patients prior to elective termination of their pregnancies. Villi were obtained in 42, and direct chromosome preparations were successful in 41 of them. The use of a mixture of 0.075 M potassium chloride and 1% sodium citrate in the ratio of 2:1 for hypotonic treatment and 40% acetic acid for cell dispersal yielded chromosomes with good morphology and G-bands.
对52例计划终止妊娠的患者进行了绒毛取样。42例成功获取绒毛，其中41例直接染色体标本制备成功。使用0.075M氯化钾与1%柠檬酸钠按2:1比例混合进行低渗处理，并使用40%乙酸进行细胞分散，得到了形态良好且具有G带的染色体。
