头部固定小鼠背侧和腹侧 CA1 神经元钙成像方案。
Protocol for calcium imaging of dorsal and ventral CA1 neurons in head-fixed mice.
机构信息
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea; Center for Synaptic Brain Dysfunctions, Institute for Basic Science, Daejeon 34141, Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea; Center for Synaptic Brain Dysfunctions, Institute for Basic Science, Daejeon 34141, Korea.
出版信息
STAR Protoc. 2023 Sep 15;4(3):102439. doi: 10.1016/j.xpro.2023.102439. Epub 2023 Jul 9.
In contrast to other techniques utilized in physiological studies, calcium imaging can visualize target neurons located deep in the brain. Here, we present a protocol for one-photon calcium imaging of dorsal and ventral CA1 neurons in head-fixed mice. We describe procedures for injecting GCaMP6f virus, implanting a gradient-index (GRIN) lens, and installing a baseplate for Inscopix microscope mounting. For complete details on the use and execution of this protocol, please refer to Yun et al..
与其他用于生理研究的技术相比，钙成像技术可以可视化位于大脑深部的目标神经元。在这里，我们介绍了一种在头部固定的小鼠中对背侧和腹侧 CA1 神经元进行单光子钙成像的方案。我们描述了注射 GCaMP6f 病毒、植入梯度折射率 (GRIN) 透镜以及安装用于 Inscopix 显微镜安装的基板的过程。有关此方案使用和执行的完整详细信息，请参阅 Yun 等人的研究。
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