使用双光子钙成像记录小鼠电刺激诱发的神经活动的方案。
Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging.
机构信息
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Institute of Ophthalmology, University College London, 11-43 Bath Street, EC1V 9EL London, UK.
出版信息
STAR Protoc. 2024 Jun 21;5(2):103027. doi: 10.1016/j.xpro.2024.103027. Epub 2024 Apr 27.
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al..
电刺激为治疗神经紊乱提供了一种可行的临床方法。在此，我们介绍了一种使用双光子钙成像记录小鼠电刺激诱发的神经活动的方案。我们详细说明了慢性植入头固定杆、刺激电极和玻璃成像窗的步骤。我们还描述了病毒注射和清醒头固定记录的过程。有关该方案使用和实施的完整详细信息，请参考 Dadarlat 等人的研究。
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