Neurobiology Section, Center for Neural Circuits and Behavior, Department of Neurosciences, and Halıcıoğlu Data Science Institute, University of California, San Diego, La Jolla, CA 92093, USA.
STAR Protoc. 2022 Apr 22;3(2):101343. doi: 10.1016/j.xpro.2022.101343. eCollection 2022 Jun 17.
Neural activity is heterogeneous across different cortical areas and can change during learning. Here, we describe a protocol for longitudinal two-photon calcium imaging with an ultra-large cranial window that exposes most of the dorsal cortex in head-fixed mice. The large cranial window allows optical access to any dorsal cortical areas in individual mice. This protocol enables longitudinal tracking of neural activity from various cortical areas at cellular resolution to understand the cortical computations during behavioral tasks. For complete details on the use and execution of this protocol, please refer to Hattori et al. (2019), and Hattori and Komiyama, 2022a.
神经活动在不同的皮质区域是不同的，并且可以在学习过程中发生变化。在这里，我们描述了一种使用超大颅窗进行纵向双光子钙成像的方案，该方案可在头固定小鼠中暴露大部分背侧皮质。大颅窗允许对单个小鼠的任何背侧皮质区域进行光学访问。该方案能够以细胞分辨率对来自各种皮质区域的神经活动进行长期跟踪，以了解行为任务过程中的皮质计算。有关此方案使用和执行的完整详细信息，请参阅 Hattori 等人。(2019 年）和 Hattori 和 Komiyama（2022a）。
