Department of Life Science, Faculty of Advanced Engineering, Chiba Institute of Technology, Narashino, Chiba, Japan.
Department of Environmental Medicine, Kochi Medical School, Kochi University, Oko-cho, Nankoku, Kochi, Japan.
PLoS One. 2023 Jul 10;18(7):e0288362. doi: 10.1371/journal.pone.0288362. eCollection 2023.
MSM/Ms mouse derived from the Japanese wild mouse has unique characteristics compared to the widely used C57BL/6 mouse. To examine the usefulness of the MSM/Ms mouse for the comparative genomic analysis, expression of small RNAs were analyzed by the large-scale sequence analysis for two strains of mouse, C57BL/6 and MSM/Ms. As a trial, expression of box C/D snoRNAs, which are the most abundant small RNAs in the cell, were analyzed. By the comparison of the read number for each fragment, 11 snoRNAs with single nucleotide polymorphisms (SNPs) were detected. One of the snoRNAs, SNORD53, shows the expression only for MSM/Ms and this snoRNA has a mutation in the box sequence in C57BL/6. Thus, it was demonstrated that the proposed experimental system using SNPs can give new insight for the gene expression regulation.
与广泛使用的 C57BL/6 小鼠相比，源自日本野生鼠的 MSM/Ms 小鼠具有独特的特征。为了检验 MSM/Ms 小鼠在比较基因组分析中的有用性，对两种小鼠（C57BL/6 和 MSM/Ms）的小 RNA 表达进行了大规模序列分析。作为一项尝试，分析了细胞中含量最丰富的小 RNA box C/D snoRNAs 的表达。通过比较每个片段的读取次数，检测到 11 个具有单核苷酸多态性（SNP）的 snoRNAs。其中一个 snoRNA，SNORD53，仅在 MSM/Ms 中表达，并且该 snoRNA 在 C57BL/6 中框序列发生突变。因此，该研究证明了使用 SNP 的建议实验系统可以为基因表达调控提供新的见解。
