Hunziker T, Nydegger U E, Lerch P G, Vassalli J D
Clin Exp Immunol. 1986 May;64(2):442-9.
Supernatant fluids were prepared from human platelets (2-2.5 X 10(10)/ml) after sonication of stimulation with cross-linked IgG; together with plasma samples from two patients with different types of pemphigus, they enhanced acantholysis in cultured punch biopsy specimens of human skin. In the absence of pemphigus plasma the platelet-derived materials did not induce acantholysis. The acantholysis-enhancing effect persisted after exhaustive dialysis or heating (56 degrees C, 30 min). The platelet-derived materials did not contain plasminogen activator; plasminogen itself was detectable by zymographic analysis, although in quantities too low (0.2-0.5 micrograms/ml) to account for the acantholysis-enhancing activity. We conclude that the platelet could contribute to the pathogenesis of pemphigus. The nature of the platelet-derived acantholysis-enhancing factors is presently unknown.
用交联IgG刺激人血小板（2 - 2.5×10¹⁰/毫升）后超声处理制备上清液；与两名不同类型天疱疮患者的血浆样本一起，它们增强了人皮肤培养打孔活检标本中的棘层松解。在没有天疱疮血浆的情况下，血小板衍生物质不会诱导棘层松解。经过彻底透析或加热（56℃，30分钟）后，棘层松解增强作用仍然存在。血小板衍生物质不含纤溶酶原激活剂；通过酶谱分析可检测到纤溶酶原本身，尽管其含量过低（0.2 - 0.5微克/毫升），无法解释棘层松解增强活性。我们得出结论，血小板可能在天疱疮的发病机制中起作用。目前尚不清楚血小板衍生的棘层松解增强因子的性质。
