Aukerman S L, Siciliano M J, Fidler I J
Clin Exp Metastasis. 1986 Jul-Sep;4(3):177-89. doi: 10.1007/BF00117931.
The major purpose of these studies was to determine whether the expression of isozymes by tumor cells was heterogeneous among tumor cell subpopulations within a neoplasm and whether expression of one or another isozyme correlated with metastatic potential of tumor cells. The expression levels of 40 isozymes were determined in 56 cell lines, many of them clonal, from nine different murine and human tumors. The enzymes chosen for study are involved in nucleotide, carbohydrate and pentose phosphate metabolism, and as such are indicators of the general metabolic and differentiational status of the cell. The tumors studied included two murine and two human malignant melanomas, four murine fibrosarcomas, and one human prostatic adenocarcinoma. The lines isolated from these tumors consisted of cells that are tumorigenic non-metastatic, tumorigenic low metastatic and tumorigenic highly metastatic. Clonally derived cell lines from a given tumor differed in their expression of a number of different isozymes, including adenosine deaminase, creatine phosphokinase-B and lactate dehydrogenase. Different patterns of isozyme expression were observed among different tumor types as well as between tumors of the same type; however, there were no differences in isozyme expression for any enzyme tested that correlated with metastatic ability of tumor cells.
这些研究的主要目的是确定肿瘤细胞中同工酶的表达在肿瘤内的肿瘤细胞亚群中是否存在异质性，以及一种或另一种同工酶的表达是否与肿瘤细胞的转移潜能相关。在来自9种不同小鼠和人类肿瘤的56个细胞系（其中许多是克隆细胞系）中测定了40种同工酶的表达水平。选择用于研究的酶参与核苷酸、碳水化合物和磷酸戊糖代谢，因此是细胞一般代谢和分化状态的指标。所研究的肿瘤包括两种小鼠和两种人类恶性黑色素瘤、四种小鼠纤维肉瘤和一种人类前列腺腺癌。从这些肿瘤中分离出的细胞系包括致瘤性非转移性、致瘤性低转移性和致瘤性高转移性的细胞。来自给定肿瘤的克隆衍生细胞系在多种不同同工酶的表达上存在差异，包括腺苷脱氨酶、肌酸磷酸激酶-B和乳酸脱氢酶。在不同肿瘤类型之间以及同一类型的肿瘤之间观察到了不同的同工酶表达模式；然而，所测试的任何酶的同工酶表达差异均与肿瘤细胞的转移能力无关。
