Fisher M S, Cifone M A
Cancer Res. 1981 Aug;41(8):3018-23.
The purpose of this study was to determine whether repeated treatment of tumor cells in vitro with mutagenic doses of ultraviolet (UV) radiation could influence the metastatic behavior of these cells in vivo. Three cloned lines of UV-2237, a fibrosarcoma induced in a C3H- mouse by chronic irradiation with UV, and SF-19, a spontaneous C3H- fibrosarcoma, were grown in culture. These cell lines varied from low to high metastatic potential as determined by in vivo tests. The cultures were exposed to UV radiation from an FS40 sunlamp at a dose that killed 40% of the cells. These UV radiation exposures were repeated at 3- to 5-day intervals for a total of 5 treatments. The mutation frequency was analyzed by monitoring the appearance of ouabain-resistant colonies following UV irradiation. With all four tumor lines, the frequency of conversion to ouabain resistance was increased more than 10-fold. Tumor cells given 5 UV radiation treatments and control cultures carried in parallel without exposure to UV radiation were tested for metastatic potential in an in vivo lung colony assay. Cell lines treated in vitro with UV radiation produced more experimental metastases than the counterpart unirradiated cultures. We conclude that, in all four tumor lines, exposure of tumorigenic cells to mutagenic doses of UV radiation can alter their biological behavior and that this may contribute to the progression of tumors from low to high metastatic capability.
本研究的目的是确定用诱变剂量的紫外线（UV）对肿瘤细胞进行体外重复处理是否会影响这些细胞在体内的转移行为。在培养中培养了三个克隆系的UV - 2237（一种通过紫外线长期照射在C3H小鼠中诱导产生的纤维肉瘤）和SF - 19（一种自发的C3H纤维肉瘤）。根据体内试验确定，这些细胞系的转移潜力从低到高各不相同。将培养物暴露于FS40太阳灯发出的紫外线辐射下，剂量为杀死40%的细胞。这些紫外线辐射暴露以3至5天的间隔重复进行，共进行5次处理。通过监测紫外线照射后哇巴因抗性菌落的出现来分析突变频率。对于所有四个肿瘤系，转化为哇巴因抗性的频率增加了10倍以上。对接受5次紫外线辐射处理的肿瘤细胞和未暴露于紫外线辐射而平行培养的对照培养物进行体内肺集落试验，以检测其转移潜力。体外经紫外线辐射处理的细胞系比未照射的对应培养物产生更多的实验性转移灶。我们得出结论，在所有四个肿瘤系中，致瘤细胞暴露于诱变剂量的紫外线辐射可改变其生物学行为，这可能有助于肿瘤从低转移能力向高转移能力发展。
