Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
School of Energy and Environmental Engineering, Hebei University of Technology, Tianjin 300401, China.
Proc Natl Acad Sci U S A. 2023 Jul 18;120(29):e2305705120. doi: 10.1073/pnas.2305705120. Epub 2023 Jul 10.
The assimilation of antibiotic resistance genes (ARGs) by pathogenic bacteria poses a severe threat to public health. Here, we reported a dual-reaction-site-modified Co/TiCT (single cobalt atoms immobilized on TiCT MXene) for effectively deactivating extracellular ARGs via peroxymonosulfate (PMS) activation. The enhanced removal of ARGs was attributed to the synergistic effect of adsorption (Ti sites) and degradation (Co-O sites). The Ti sites on Co/TiCT nanosheets bound with PO on the phosphate skeletons of ARGs via Ti-O-P coordination interactions, achieving excellent adsorption capacity (10.21 × 10 copies mg) for and the Co-O sites activated PMS into surface-bond hydroxyl radicals (•OH), which can quickly attack the backbones and bases of the adsorbed ARGs, resulting in the efficient in situ degradation of ARGs into inactive small molecular organics and NO. This dual-reaction-site Fenton-like system exhibited ultrahigh extracellular ARG degradation rate (k > 0.9 min) and showed the potential for practical wastewater treatment in a membrane filtration process, which provided insights for extracellular ARG removal via catalysts design.
抗生素耐药基因（ARGs）被致病菌同化，对公共健康构成严重威胁。在这里，我们报道了一种双反应位点修饰的 Co/TiCT（单钴原子固载在 TiCT MXene 上），可通过过一硫酸盐（PMS）活化有效灭活细胞外 ARGs。增强的 ARGs 去除归因于吸附（Ti 位点）和降解（Co-O 位点）的协同作用。Co/TiCT 纳米片上的 Ti 位点通过 Ti-O-P 配位相互作用与 ARGs 磷酸盐骨架上的 PO 结合，对 表现出优异的吸附能力（10.21×10 拷贝 mg），而 Co-O 位点则将 PMS 激活为表面键合的羟基自由基（•OH），可以快速攻击吸附的 ARGs 的骨架和碱基，将 ARGs 高效原位降解为非活性小分子有机物和 NO。这种双反应位点类 Fenton 体系表现出超高的细胞外 ARG 降解速率（k > 0.9 min），并在膜过滤过程中显示出实际废水处理的潜力，为通过催化剂设计去除细胞外 ARG 提供了思路。
