硬骨鱼的中枢神经系统髓磷脂：通过染色和免疫印迹对其蛋白质进行比较电泳分析。

Central nervous system myelin of teleosts: comparative electrophoretic analysis of its proteins by staining and immunoblotting.

作者信息

Waehneldt T V, Stoklas S, Jeserich G, Matthieu J M

出版信息

Comp Biochem Physiol B. 1986;84(3):273-8. doi: 10.1016/0305-0491(86)90076-3.

Abstract

CNS myelin was isolated from 24 teleostean fishes and the proteins were analyzed by staining and immunoblotting. All species showed a 36 K protein, two or more glycosylated hydrophobic intermediate protein (IP) components and several myelin basic protein bands (BP). The 36 K protein was specific for teleostean fishes. The IP and BP components displayed substantial variations in their proportions as well as in molecular sizes when comparing the different teleosts. This contrasts with CNS myelin proteins which appear more stable in terrestrial vertebrates.

摘要

从24种硬骨鱼中分离出中枢神经系统髓磷脂，并通过染色和免疫印迹分析其蛋白质。所有物种均显示出一种36K蛋白、两种或更多种糖基化疏水中间蛋白（IP）成分以及几条髓鞘碱性蛋白带（BP）。36K蛋白是硬骨鱼特有的。在比较不同硬骨鱼时，IP和BP成分在比例以及分子大小上表现出显著差异。这与陆生脊椎动物中似乎更稳定的中枢神经系统髓磷脂蛋白形成对比。