少量中枢神经组织髓磷脂的制备与分析：颤抖小鼠的区域研究

The preparation and analysis of myelin from small quantities of central nervous tissue: regional studies of the quaking mouse.

作者信息

Fagg G E, Waehneldt T V, Neuhoff V

出版信息

Adv Exp Med Biol. 1978;100:135-45. doi: 10.1007/978-1-4684-2514-7_9.

Abstract

Myelin of considerable purity may be isolated from small (minimum 1 mg wet weight) samples of central nervous tissue, using a 4-step centrifugation procedure. The separation of myelin proteins by micro-linear gradient polyacrylamide gel electrophoresis yields similar results to those obtained by macro-scale (homogeneous) gel systems. These techniques have been employed for a preliminary study of the regional composition of myelin fractions from the Quaking mouse.

摘要

利用四步离心程序，可从少量（最低湿重1毫克）中枢神经组织样本中分离出纯度相当高的髓磷脂。通过微线性梯度聚丙烯酰胺凝胶电泳分离髓磷脂蛋白，所得结果与通过宏观规模（均匀）凝胶系统获得的结果相似。这些技术已用于对颤抖小鼠髓磷脂组分区域组成的初步研究。