Diemer Etienne, Chadni Morad, Grimi Nabil, Ioannou Irina
URD Agro-Biotechnologie Industrielles (ABI), Centre Européen de Biotechnologie et Bioéconomie (CEBB), AgroParisTech, CEDEX, 51110 Pomacle, France.
Transformations Intégrées de la Matière Renouvelable (TIMR), Centre de Recherche Royallieu-CS 60319, ESCOM, Université de Technologie de Compiègne, CEDEX, 60203 Compiègne, France.
Foods. 2022 Oct 14;11(20):3214. doi: 10.3390/foods11203214.
Forced chicory roots (FCR) are the main but also the least valued by-products of Belgian endive culture. However, they contain molecules of interest for industry such as caffeoylquinic acids (CQAs). This study aims to investigate accelerated solvent extraction (ASE) as a green technique to recover chlorogenic acid (5-CQA) and 3,5-dicaffeoylquinic acid (3,5-diCQA), the main CQAs. A D-optimal design was used to determine the influence of temperature and ethanol percentage on their extraction. Optimal extraction conditions were determined using response surface methodology (RSM) and allow the recovery of 4.95 ± 0.48 mg/g of 5-CQA at 107 °C, 46% of ethanol and 5.41 ± 0.79 mg/g of 3,5-diCQA at 95 °C, 57% of ethanol. The antioxidant activity of the extracts was also optimized by RSM. The highest antioxidant activity was achieved at 115 °C with 40% ethanol (more than 22mgTrolox/g). Finally, correlation between the antioxidant activity and the amount of CQAs was determined. FCR can be a great source of bioactive compounds with potential use as biobased antioxidant.
强制菊苣根（FCR）是比利时苣荬菜种植的主要副产品，但也是最不受重视的副产品。然而，它们含有对工业有价值的分子，如咖啡酰奎宁酸（CQAs）。本研究旨在研究加速溶剂萃取（ASE）作为一种绿色技术来回收主要的CQAs，即绿原酸（5-CQA）和3,5-二咖啡酰奎宁酸（3,5-diCQA）。采用D-最优设计来确定温度和乙醇百分比对其萃取的影响。使用响应面法（RSM）确定了最佳萃取条件，在107℃、46%乙醇条件下可回收4.95±0.48mg/g的5-CQA，在95℃、57%乙醇条件下可回收5.41±0.79mg/g的3,5-diCQA。提取物的抗氧化活性也通过RSM进行了优化。在115℃、40%乙醇条件下获得了最高的抗氧化活性（超过22mg Trolox/g）。最后，确定了抗氧化活性与CQAs含量之间的相关性。FCR可以成为具有潜在生物基抗氧化剂用途的生物活性化合物的重要来源。
