Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstraße 48, 48149 Münster, Germany.
Department of Chemistry and Pharmacy, Friedrich-Alexander-Universität Erlangen-Nürnberg, Nikolaus-Fiebiger-Str. 10, 91058 Erlangen, Germany.
J Agric Food Chem. 2023 Jul 26;71(29):11263-11275. doi: 10.1021/acs.jafc.3c02073. Epub 2023 Jul 11.
Cereals contain lipids that fulfill important physiological roles and are associated with stress in the plant. However, many of the specific biological roles of lipids are yet unknown. Comprehensive analysis of these polar lipid categories in whole grain wheat and oat, cereals highly relevant also in nutrition, was performed. Hydrophilic interaction liquid chromatography (HILIC) and reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with high-resolution mass spectrometry using electrospray ionization in both positive and negative ionization mode was used. Exploiting the different separation mechanisms, HILIC was used as a screening method for straightforward lipid class assignment and enabled differentiation of isomeric lipid classes, like phosphatidylethanolamine and lyso--acylphosphatidylethanolamine, while RP-HPLC facilitated separation of constitutional isomers. In combination with data-dependent MS/MS experiments, 67 lipid species belonging to nine polar lipid classes could be identified. Furthermore, with both ionization modes, fatty acyl chains directly connected to the lipid headgroups could be assigned. This work focused on the four lipid classes -acylphosphatidylethanolamines, acyl-monogalactosyldiacylglycerols, digalactosyldiacylglycerols, and monogalactosyldiacylglycerols as they were less studied in detail in the past. Applying the complementary approach, the relative lipid species compositions in these lipid classes was investigated in detail
谷物含有脂质，这些脂质具有重要的生理功能，与植物应激有关。然而，许多脂质的具体生物学功能仍然未知。本研究对全谷物小麦和燕麦中这些极性脂质类别进行了综合分析，这两种谷物在营养方面也非常重要。采用亲水相互作用液相色谱（HILIC）和反相高效液相色谱（RP-HPLC），结合正、负离子模式下的电喷雾电离高分辨质谱，对这些脂质进行了分析。利用不同的分离机制，HILIC 可作为一种简单的脂质分类分配筛选方法，并能够区分类似的脂质类，如磷脂酰乙醇胺和溶血磷脂酰乙醇胺，而 RP-HPLC 则有利于结构异构体的分离。结合数据依赖的 MS/MS 实验，共鉴定出 67 种属于 9 种极性脂质类别的脂质。此外，通过两种离子模式，可直接对连接到脂质头部的脂肪酸链进行分配。本研究重点关注四个脂质类别的脂质，即酰基磷脂酰乙醇胺、酰基单半乳糖基二酰甘油、双半乳糖基二酰甘油和单半乳糖基二酰甘油，因为过去对它们的研究较少。应用互补的方法，详细研究了这些脂质类别的相对脂质种类组成。
