Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, School of Pharmacy, Anhui Medical University, Hefei 230032, People's Republic of China.
Nanotechnology. 2023 Jul 27;34(41). doi: 10.1088/1361-6528/ace633.
We describes the development of a self-assembled nanoprobe for ratiometric sensing of hypoxia in living cells. The probe, UC-AuNPs, is composed of azo-functionalized upconversion nanoparticles (azo-UCNPs) and gold nanoparticles functionalized with-cyclodextrin (CD-AuNPs). Under hypoxic conditions, reductases reduce azo derivatives on the UCNPs, leading to detachment of the CD-AuNPs and subsequent fluorescence recovery of the green emission. The ratiometric measurement incorporated into the strategy reduces the impact of external factors and improves sensitivity of the probe. The use of NIR excitation effectively minimizes interference from strong luminescence backgrounds in biosystems. The UC-AuNPs nanoprobe is able to effectively sense and monitor hypoxia conditions in living cells and has the potential to distinguish hypoxia-related diseases from healthy tissue, making it a valuable tool for early clinical diagnosis.
我们描述了一种用于活细胞内缺氧比色感应的自组装纳米探针的开发。该探针 UC-AuNPs 由偶氮功能化的上转换纳米粒子（azo-UCNPs）和金纳米粒子组成，这些金纳米粒子被-cyclodextrin（CD-AuNPs）功能化。在缺氧条件下，还原酶还原 UCNPs 上的偶氮衍生物，导致 CD-AuNPs 脱离，随后绿色发射的荧光恢复。该策略中的比率测量减少了外部因素的影响，提高了探针的灵敏度。近红外激发的使用有效地最小化了生物系统中强荧光背景的干扰。UC-AuNPs 纳米探针能够有效地感应和监测活细胞中的缺氧情况，并有可能将与缺氧相关的疾病与健康组织区分开来，使其成为早期临床诊断的有价值工具。
