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用噬菌体λdilv对大肠杆菌K-12的ilvO基因座进行定位

Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage lambda dilv.

作者信息

Patin D W, Calhoun D H

出版信息

J Bacteriol. 1979 Mar;137(3):1234-42. doi: 10.1128/jb.137.3.1234-1242.1979.

Abstract

A set of lambda dilv phage have been used in a deletion mapping procedure to determine the location of two previously characterized ilvO alleles. In contrast to earlier conclusions derived from three-factor crosses and episome-shortening techniques with phage P1, the order found is ilvG-ilvO-ilvEDA. A three-factor cross with phage P1 is described that is not consistent with this location for an ilvO allele. Further analysis of this particular three-factor cross revealed than an artifact attributable to a mutual syntrophism had skewed the apparent frequency of inheritance of the ilvO locus. The role of mutual syntrophism is discussed as a source of mapping errors for the ilvO locus. The value of this set of lambda dilv phage and this mapping procedure for obtaining comparatively unambiguous data on the locations of the ilv structural and regulatory genes is demonstrated.

摘要

一组λdilv噬菌体已用于缺失作图程序,以确定两个先前已鉴定的ilvO等位基因的位置。与早期通过噬菌体P1的三因子杂交和附加体缩短技术得出的结论相反,发现的顺序是ilvG-ilvO-ilvEDA。描述了一个与ilvO等位基因的这个位置不一致的噬菌体P1三因子杂交。对这个特定三因子杂交的进一步分析表明,由于相互营养缺陷型导致的假象扭曲了ilvO基因座的明显遗传频率。讨论了相互营养缺陷型作为ilvO基因座作图错误来源的作用。证明了这组λdilv噬菌体和这个作图程序对于获得关于ilv结构和调控基因位置的相对明确数据的价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a223/218306/3671b9e8c018/jbacter00286-0186-a.jpg

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