大肠杆菌ilvB基因座的一个新图谱定位。
A new map location for the ilvB locus of Escherichia coli.
作者信息
Newman T C, Levinthal M
出版信息
Genetics. 1980 Sep;96(1):59-77. doi: 10.1093/genetics/96.1.59.
Abstract
We isolated, in E. coli K12, new alleles of the ilvB locus, the structural gene for acetolactate synthase isoenzyme I, and showed them to map at or near the ilvB619 site. The map position of the ilvB locus was redetermined because plasmids containing the ilvC-cya portion of the chromosome did not complement mutations at the ilvB locus. Furthermore, diploids for the ilvEDAC genes formed with these plasmids in an ilvHI background facilitated the isolation of the new ilvB alleles. The ilvB locus was remapped and found to be located at 81.5 minutes, between the uhp and dnaA loci. This location was determined by two- and three-point transductional crosses, deletion mapping and complementation with newly isolated plasmids. One of the new alleles of the ilvB gene is a mu-1 insertion. When present in the donor strain, this allele interferes with the linkage of genes flanking the mu-1 insertion, as well as the linkage of genes to either side of the mu-1 insertion.
摘要
我们在大肠杆菌K12中分离出乙酰乳酸合酶同工酶I的结构基因ilvB位点的新等位基因，并证明它们定位于ilvB619位点或其附近。由于含有染色体ilvC-cya部分的质粒不能互补ilvB位点的突变，因此重新确定了ilvB位点的图谱位置。此外，在ilvHI背景下用这些质粒形成的ilvEDAC基因的二倍体有助于新ilvB等位基因的分离。对ilvB位点进行了重新定位，发现它位于uhp和dnaA位点之间的81.5分钟处。这个位置是通过两点和三点转导杂交、缺失图谱分析以及与新分离的质粒互补来确定的。ilvB基因的一个新等位基因是一个mu-1插入。当存在于供体菌株中时，这个等位基因会干扰mu-1插入侧翼基因的连锁，以及mu-1插入两侧基因的连锁。
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