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ilv-lac融合体的物理特性分析。

Physical characterization of ilv-lac fusions.

作者信息

Leathers T D, Noti J, Umbarger H E

出版信息

J Bacteriol. 1979 Oct;140(1):251-60. doi: 10.1128/jb.140.1.251-260.1979.

DOI:10.1128/jb.140.1.251-260.1979
PMID:159287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216803/
Abstract

Electron microscopic heteroduplex analysis and comparative restriction digests have been used to characterize lambda p123(209), the complementary pair of phages used in the Casadaban technique of gene fusion. Derivatives of lambda 1(209) constructed to carry fusions of the lac genes to the control regions of the ilvC and ilvEDA operons were also analyzed. These physical maps have provided confirmation of the genetic models for these constructions and physical specifications important in interpreting the behavior of these ilv-lac fusions.

摘要

电子显微镜异源双链分析和比较限制性酶切已被用于表征λ p123(209)，这是在卡萨达班基因融合技术中使用的互补噬菌体对。还分析了构建的携带lac基因与ilvC和ilvEDA操纵子控制区融合的λ 1(209)衍生物。这些物理图谱证实了这些构建的遗传模型以及对解释这些ilv-lac融合行为很重要的物理特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/216803/3c959c43e911/jbacter00275-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/216803/3c959c43e911/jbacter00275-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/216803/3c959c43e911/jbacter00275-0262-a.jpg

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引用本文的文献

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本文引用的文献

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2
Isolation of transducing bacteriophages for the histidine and isoleucine-valine operons in Escherichia coli K-12.大肠杆菌K-12中组氨酸和异亮氨酸-缬氨酸操纵子转导噬菌体的分离
J Bacteriol. 1972 Oct;112(1):40-7. doi: 10.1128/jb.112.1.40-47.1972.
3
Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.用特异性核酸内切酶分析噬菌体μ及λ-μ杂种DNA
大肠杆菌磷酸己糖转运系统表达的正向激活因子的证据及uhp区域的分子克隆
J Bacteriol. 1983 Sep;155(3):1062-70. doi: 10.1128/jb.155.3.1062-1070.1983.
4
Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.λplacMu：一种噬菌体λ的可转座衍生物，用于一步构建lacZ蛋白融合体。
J Bacteriol. 1984 Jun;158(3):1084-93. doi: 10.1128/jb.158.3.1084-1093.1984.
5
Construction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.利用携带启动子 - lac融合的λ噬菌体的Mu d1（lac Apr）溶原菌构建：λ ppheA - lac的分离
J Bacteriol. 1982 Jun;150(3):1122-9. doi: 10.1128/jb.150.3.1122-1129.1982.
6
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J Bacteriol. 1982 Mar;149(3):1071-81. doi: 10.1128/jb.149.3.1071-1081.1982.
7
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9
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10
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