Abdelraof Mohamed, Fikry Mohamed, Hashem Amr H, El-Naggar Mehrez E, Rashdan Huda R M
Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre Dokki Cairo 12622 Egypt.
Ultrafast Picosecond Laser Lab, Physics Department, Faculty of Science, Cairo University Giza 12613 Egypt
RSC Adv. 2023 Jul 10;13(30):20684-20697. doi: 10.1039/d3ra02775a. eCollection 2023 Jul 7.
For drug delivery applications, silica nanoemulsion encapsulated with organic compounds are becoming increasingly more desirable. Therefore, the emphasis of this research was on the synthesis of a new potent antifungal drug-like candidate (1,1'-((sulfonylbis(4,1-phenylene)bis(5-methyl-1-1,2,3-triazole-1,4-diyl))bis(3-(dimethylamino)prop-2-en-1-one), SBDMP), the chemical structure of which was confirmed on the basis of its spectral and microanalytical data. Then, silica nanoemulsion loaded with SBDMP was prepared using Pluronic F-68 as a potent surfactant. The particle shape, hydrodynamic size, and zeta potential of the produced silica nanoemulsion (with and without drug loading) were assessed. The antitumoral activity of the synthesized molecules showed the superiority of SBDMP and silica nanoemulsion with and without SBDMP loading against and . Subsequently, the laser-induced photodynamic inactivation (LIPDI) of Mucorales strains was determined using the tested samples. The optical properties of the samples were investigated using UV-vis optical absorption and the photoluminescence. The photosensitivity of the selected samples appeared to enhance the eradication of the tested pathogenic strains when exposed to a red (640 nm) laser light. The optical property results verified that the SBDMP-loaded silica nanoemulsion has a high depth of penetration into biological tissues due to a two-absorption photon (TAP) mechanism. Interestingly, the photosensitizing of the nanoemulsion loaded with a newly synthesized drug-like candidate, SBDMP, opens up a new route to apply new organic compounds as photosensitizers under laser-induced photodynamic therapy (LIPDT).
对于药物递送应用而言，包裹有机化合物的二氧化硅纳米乳剂越来越受到青睐。因此，本研究的重点是合成一种新型强效抗真菌类候选药物（1,1'-((磺酰基双(4,1-亚苯基)双(5-甲基-1H-1,2,3-三唑-1,4-二基))双(3-(二甲氨基)丙-2-烯-1-酮)，SBDMP），其化学结构已根据光谱和微量分析数据得到确认。然后，以普朗尼克F-68作为强效表面活性剂制备了负载SBDMP的二氧化硅纳米乳剂。对所制备的二氧化硅纳米乳剂（有载药和无载药）的颗粒形状、流体动力学尺寸和zeta电位进行了评估。合成分子的抗肿瘤活性表明，SBDMP以及负载和未负载SBDMP的二氧化硅纳米乳剂对[具体肿瘤细胞名称缺失]和[具体肿瘤细胞名称缺失]具有优越性。随后，使用测试样品测定了毛霉目菌株的激光诱导光动力失活（LIPDI）。通过紫外-可见光学吸收和光致发光研究了样品的光学性质。当暴露于红色（640 nm）激光时，所选样品的光敏性似乎增强了对测试致病菌株的根除效果。光学性质结果证实，负载SBDMP的二氧化硅纳米乳剂由于双光子吸收（TAP）机制而具有高组织穿透深度。有趣的是，负载新合成的类药物候选物SBDMP的纳米乳剂的光敏化开辟了一条在激光诱导光动力疗法（LIPDT）下将新有机化合物用作光敏剂的新途径。
