Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, PR China.
Photodiagnosis Photodyn Ther. 2013 Dec;10(4):460-9. doi: 10.1016/j.pdpdt.2013.04.002. Epub 2013 Jun 12.
Nanoparticles have been explored recently as an efficient means to deliver photosensitizers for photodynamic therapy. However, it is largely unknown if polyhematoporphyrin (C34H38N4NaO5, Photosan-II, PS) or other photosensitizers can be efficiently delivered by hollow silica nanoparticles (HSNP).
Polyhematoporphyrin (C34H38N4NaO5, Photosan-II, PS) was loaded into hollow silica nanoparticles (HSNP) by one-step wet chemical-based synthetic route. Dynamic light scattering (DLS) and polydispersive index (PDI) were used for measurement of the particles size and size distribution. Transmission electron microscope and scanning electron microscopy were used for the microstructure, morphological and chemical composition analysis. Fourier transform infrared spectrometry spectra and fluorescence emission spectrum were obtained. The photobiological activity of the PS-loaded HSNP was evaluated on human cholangiocarcinoma QBC939 cells. The cellular viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Apoptotic and necrotic cells were measured by flow cytometry.
DLS measurements showed that the size of the particles is in the range of 25-90 nm. PDI of the PS-loaded HSNP is 0.121 ± 0.01, indicating that samples have excellent quality with narrow size distribution to monomodal systems. In MTT assay, PS-loaded HSNP and free PS of the same concentration killed about 95.3% ± 2.0% and 55.7% ± 1.9% of QBC939 cells, respectively. The flow cytometry demonstrated that the laser induced cell death with PS-loaded HSNP was much more severe than that of free PS (P<0.05).
Photosan-II-loaded hollow silica nanoparticles not only can quickly deliver Photosan-II into cells but also can reach a more high concentration than free Photosan-II. HSNP is a desirable vehicle and the release system that shows promises for photodynamic therapy use, which not only improve the aqueous solubility, stability and transport efficiency of PS, but also increase its photodynamic efficacy compared to free PS.
最近，纳米粒子已被探索用于传递光敏剂以进行光动力疗法，这是一种有效的方法。然而，目前尚不清楚多血卟啉（C34H38N4NaO5，Photosan-II，PS）或其他光敏剂是否可以被中空二氧化硅纳米粒子（HSNP）有效传递。
通过一步湿化学合成路线将多血卟啉（C34H38N4NaO5，Photosan-II，PS）载入中空二氧化硅纳米粒子（HSNP）中。动态光散射（DLS）和多分散指数（PDI）用于测量粒子的尺寸和尺寸分布。透射电子显微镜和扫描电子显微镜用于微观结构、形态和化学成分分析。傅里叶变换红外光谱和荧光发射光谱。在人胆管癌细胞 QBC939 上评价负载 PS 的 HSNP 的光生物活性。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）测定法测定细胞活力。通过流式细胞术测量凋亡和坏死细胞。
DLS 测量结果表明，粒子的尺寸在 25-90nm 范围内。PS 负载的 HSNP 的 PDI 为 0.121±0.01，表明样品具有极好的质量，具有窄的尺寸分布，为单模态系统。在 MTT 测定中，相同浓度的负载 PS 的 HSNP 和游离 PS 分别杀死约 95.3%±2.0%和 55.7%±1.9%的 QBC939 细胞。流式细胞术表明，负载 PS 的 HSNP 诱导的激光细胞死亡比游离 PS 更严重（P<0.05）。
负载 Photosan-II 的中空二氧化硅纳米粒子不仅可以快速将 Photosan-II 递送到细胞内，而且可以达到比游离 Photosan-II 更高的浓度。HSNP 是一种理想的载体和释放系统，有望用于光动力疗法，不仅提高了 PS 的水溶性、稳定性和输送效率，而且与游离 PS 相比，提高了其光动力疗效。
