Department of Biology, University of Ottawa, Ottawa, ON, Canada.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
Front Endocrinol (Lausanne). 2023 Jun 23;14:1225199. doi: 10.3389/fendo.2023.1225199. eCollection 2023.
Aromatase (Cyp19a1) is the steroidogenic enzyme that converts androgens into bioactive estrogens, and hence is in a pivotal position to mediate reproduction and sexual behavior. In teleosts, there are two aromatase paralogs: that is highly expressed in granulosa and Leydig cells in the gonads with critical function in sexual differentiation of the ovary, and that is highly expressed in radial glial cells in the brain with unknown roles in reproduction. mutant zebrafish lines were used to investigate the importance of the paralogs for spawning behavior and offspring survival and early development. Mutation of was found to increase the latency to the first oviposition in females. Mutation of in females also increased the number of eggs spawned; however, significantly more progeny died during early development resulting in no net increase in female fecundity. This finding suggests a higher metabolic cost of reproduction in mutant females. In males, the combined mutation of both paralogs resulted in significantly lower progeny survival rates, indicating a critical function of during early larval development. These data establish the specific importance of for female spawning behavior and the importance of the paralogs for early larval survival.
芳香酶（Cyp19a1）是一种甾体生成酶，可将雄激素转化为生物活性雌激素，因此在介导生殖和性行为方面处于关键地位。在硬骨鱼中，有两种芳香酶基因的同源异构体： ，在性腺中的颗粒细胞和间质细胞中高度表达，对卵巢的性别分化具有关键作用； ，在大脑中的放射状胶质细胞中高度表达，其在生殖中的作用尚不清楚。 使用 突变斑马鱼系来研究这两个同源异构体在产卵行为和后代存活及早期发育中的重要性。发现 突变会增加雌性鱼类的首次产卵潜伏期。 突变的雌性鱼类产卵数量也有所增加；然而，在早期发育过程中有更多的后代死亡，导致雌性鱼类的繁殖力没有净增加。这一发现表明 突变的雌性鱼类的繁殖代谢成本更高。在雄性鱼类中，两个 同源异构体的联合突变导致后代的存活率显著降低，表明 在早期幼虫发育过程中具有关键作用。这些数据确立了 对雌性鱼类产卵行为的特殊重要性，以及 同源异构体对早期幼虫存活的重要性。
