Sidiki Ngouyamsa Nsapkain Aboubakar, Nadia Noumedem Anangmo Christelle, Cedric Yamssi, Guy-Armand Gamago Nkadeu, Sandra Tientcheu Noutong Jemimah, Kevin Tako Djimefo Alex, Azizi Mounvera Abdel, Payne Vincent Khan
Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067 Dschang, Cameroon.
Department of Microbiology, Hematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96 Dschang, Cameroon.
J Parasitol Res. 2023 Jul 3;2023:3350293. doi: 10.1155/2023/3350293. eCollection 2023.
Reduction of oxidative stress during malaria infection is considered as being of great benefit so long as treatment and drug development approaches are concerned. This study had the aim of evaluating the antimalarial and antioxidant activities of the ethanolic extract of in Swiss albino mice infected with the NK65 strain.
the antiplasmodial activity of the plant ethanolic extract was tested in a four-day suppressive and curative assay using in Swiss albino mice. The extract was administered to the mice at doses of 125, 250, and 500 mg/kg per day. Then, parameters, such as parasite suppression and survival time of the mice, were evaluated. Furthermore, the effect of plant extract on liver damage, oxidative stress indicators, and lipid profile changes in -infected mice were studied.
Administration of significantly suppressed infection by 55.17%, 70.69%, and 71.10% at doses of 125, 250, and 500 mg/kg, respectively, whereas chloroquine had 84.64% suppression relative to the untreated group 1% Dimethyl sulfoxide (1% DMSO) at day 4 (post-infection) in the four-day suppressive test. This suppression activity rate was dose-dependent. The curative test also presented a significant reduction in parasitemia and an extension of the survival time of the treated groups. Treatment of infected parasitized mice with the extract of had a significant ( < 0.05) reduction in parameters, such as total protein, aspartate aminotransferase, and alanine aminotransferase. Infection may also lead to a significant increase in the enzymatic activity of liver catalase and superoxide dismutase compared with the normal control group. The non-enzymatic antioxidant activity in parasitized mice was significantly reduced in malondialdehyde and increased in glutathione and nitric oxide when compared with the normal control group.
These findings support the ethnobotanical use of stem bark as an antimalarial remedy coupled with antioxidant activity. However, further toxicity tests are required to ascertain its safety.
就疟疾感染的治疗和药物研发方法而言，降低疟疾感染期间的氧化应激被认为具有极大益处。本研究旨在评估[植物名称]乙醇提取物对感染NK65株疟原虫的瑞士白化小鼠的抗疟和抗氧化活性。
使用感染疟原虫的瑞士白化小鼠，通过为期四天的抑制和治愈试验来测试该植物乙醇提取物的抗疟活性。提取物以每天125、250和500mg/kg的剂量给予小鼠。然后，评估诸如寄生虫抑制和小鼠存活时间等参数。此外，研究了植物提取物对感染疟原虫小鼠的肝损伤、氧化应激指标和脂质谱变化的影响。
在为期四天的抑制试验中，在感染后第4天，[植物名称]提取物分别以125、250和500mg/kg的剂量给药时，对疟原虫感染的抑制率分别为55.17%、70.69%和71.10%，而氯喹相对于未处理组（1%二甲基亚砜）的抑制率为84.64%。这种抑制活性率呈剂量依赖性。治愈试验也显示治疗组的疟原虫血症显著降低，存活时间延长。用[植物名称]提取物治疗感染疟原虫的小鼠，总蛋白、天冬氨酸转氨酶和丙氨酸转氨酶等参数显著降低（P<0.05）。与正常对照组相比，感染还可能导致肝脏过氧化氢酶和超氧化物歧化酶的酶活性显著增加。与正常对照组相比，感染疟原虫小鼠的非酶抗氧化活性在丙二醛中显著降低，在谷胱甘肽和一氧化氮中增加。
这些发现支持了将[植物名称]茎皮作为具有抗氧化活性抗疟药物的民族植物学用途。然而，需要进一步进行[植物名称]毒性试验以确定其安全性。
