Cédric Yamssi, Nadia Noumedem Anangmo Christelle, Nfufu Sandra, Azizi Mounvera Abdel, Sandra Tientcheu Noutong Jemimah, Payne Vincent Khan
Department of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39 Bambili, Cameroon.
Department of Microbiology, Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96 Dschang, Cameroon.
J Parasitol Res. 2023 Sep 14;2023:9545565. doi: 10.1155/2023/9545565. eCollection 2023.
Infections with gastrointestinal helminths constitute a serious obstacle to the good health of the local population in most African Countries. The aim of this study was to evaluate the anthelminthic activity of ethanol and aqueous extracts against using the worm microtracker.
Aqueous and ethanolic extracts of were prepared. Different concentrations of the extracts were tested against the egg and larvae stages of using an automated high-throughput method. Briefly, embryonated eggs and larvae of this parasite were obtained after the incubation of fresh eggs at 25°C for 24, 48, and 96 hours for embryonated eggs, L and L larvae, respectively. One hundred microliters of the plant extracts at various concentrations were put in contact in a 96-well microplate with a suspension of 100 embryonated eggs in a total volume of 200 L and incubated in a worm microtracker where the motility of the worms was recorded every 30 minutes for the ovicidal activity. The final tested extract concentration was 5, 2.5, 1.25, 0.625, and 0.3125 mg/mL, whereas ringer solution (0.95%) and 1.5% Dimethyl sulfoxide (DMSO) were used as negative controls and levamisole as positive control. The same method was used for larvicidal activities. The anthelmintic activity was determined using the average movement of the worms in the tested product compared with the negative control (1.5% DMSO and ringer solution).
The egg hatching rates of had IC of 0.49 mg/mL (95% confidence interval: 71.70-92.03) and 0.22 mg/mL (95% confidence interval: 74.28-86.18) for the ethanol and aqueous extract, respectively. These IC indicate that the aqueous extract is more active for the inhibition of hatching at a 95% confidence interval. The aqueous and ethanol extracts presented mean inhibitory hatching rates of 78.33 ± 1.67% and 75.67 ± 1.15% at 5 mg/mL, respectively, with no significant differences. The highest percentage of inhibition of L larva was observed at 5 mg/mL with 89 ± 2.3%and 85 ± 2.7% for the ethanol and aqueous extracts, respectively. The lowest percentage of inhibition was observed at 0.3125 mg/mL, with 54.67 ± 3.38% and 49 ± 2.64% for the ethanol and aqueous extract, respectively. No significant differences were observed between the two extracts at 5 mg/mL with an inhibitory percentage of 90.67 ± 3.05% (ethanol) and 89.33 ± 2.08% (aqueous).
Extracts of seeds possess nematocidal activity, however, further and investigations are necessary to confirm their anthelminthic activity.
在大多数非洲国家，胃肠道蠕虫感染是当地居民健康的严重障碍。本研究的目的是使用蠕虫微跟踪器评估乙醇提取物和水提取物的驱虫活性。
制备了[植物名称]的水提取物和乙醇提取物。使用自动高通量方法测试不同浓度的提取物对[寄生虫名称]的卵和幼虫阶段的作用。简而言之，分别在25°C下将新鲜卵孵育24、48和96小时后获得该寄生虫的胚胎卵和幼虫，分别为L和L幼虫。将100微升不同浓度的植物提取物与100个胚胎卵的悬浮液在96孔微孔板中接触，总体积为200微升，并在蠕虫微跟踪器中孵育，每30分钟记录一次蠕虫的运动以进行杀卵活性测试。最终测试的提取物浓度为5、2.5、1.25、0.625和0.3125毫克/毫升，而林格氏液（0.95%）和1.5%二甲亚砜（DMSO）用作阴性对照，左旋咪唑用作阳性对照。幼虫杀灭活性采用相同方法。与阴性对照（1.5%DMSO和林格氏液）相比，使用测试产品中蠕虫的平均运动来确定驱虫活性。
[寄生虫名称]的卵孵化率，乙醇提取物的半数抑制浓度（IC）为0.49毫克/毫升（95%置信区间：71.70 - 92.03），水提取物的IC为0.22毫克/毫升（95%置信区间：74.28 - 86.18）。这些IC表明水提取物在95%置信区间对孵化抑制更具活性。水提取物和乙醇提取物在5毫克/毫升时的平均抑制孵化率分别为78.33±1.67%和75.67±1.15%，无显著差异。在5毫克/毫升时观察到对L幼虫的抑制百分比最高，乙醇提取物和水提取物分别为89±2.3%和85±2.7%。在0.3125毫克/毫升时观察到的抑制百分比最低，乙醇提取物和水提取物分别为54.67±3.38%和49±2.64%。在5毫克/毫升时，两种提取物之间未观察到显著差异，抑制百分比分别为90.67±3.05%（乙醇）和89.33±2.08%（水）。
[植物名称]种子提取物具有杀线虫活性，然而，需要进一步的[具体研究类型1]和[具体研究类型2]研究来证实其驱虫活性。
