茎皮水提取物对感染……的小鼠的抗疟活性
Antiplasmodial Activity of Stem Bark Aqueous Extract in Mice Infected by .
作者信息
Tchatat Tali Mariscal Brice, Jiatsa Mbouna Cedric Derick, Yamthe Tchokouaha Lauve Rachel, Tsouh Fokou Patrick Valere, Tsakem Nangap Jaures Marius, Keumoe Rodrigue, Ngoutane Mfopa Alvine, Bakarnga-Via Issakou, Gounoue Kamkumo Raceline, Fekam Boyom Fabrice
机构信息
Antimicrobial & Biocontrol Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, Cameroon.
Institute for Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, PO Box 6163, Yaoundé, Cameroon.
出版信息
J Parasitol Res. 2020 Jun 29;2020:4580526. doi: 10.1155/2020/4580526. eCollection 2020.
BACKGROUND
is used in Cameroon traditional medicine to treat malaria and related symptoms. However, its antiplasmodial efficacy is still to be established.
OBJECTIVES
The present study is aimed at evaluating the and antiplasmodial activity and the oral acute toxicity of the extracts.
MATERIALS AND METHODS
Extracts were prepared from leaves and stem bark of , by maceration in distilled water, methanol, ethanol, dichloromethane (DCM), and hexane. All extracts were initially screened against the chloroquine-resistant strain W2 of to confirm its activity, and the most potent one was assessed in malaria mouse model at three concentrations (100, 200, and 400 mg/kg/bw). Biochemical, hematological, and histological parameters were also determined.
RESULTS
Overall, 7 extracts showed antiplasmodial activity with IC ranging from 0.809 g/mL to 5.886 g/mL. The aqueous extract from the stem bark of (sb) was the most potent (IC = 0.809 g/mL) and was further assessed for acute toxicity and efficacy in -infected mice. sb was safe in mice with a median lethal dose (LD) higher than 2000 mg/kg of body weight. It also exerted a good antimalarial efficacy with ED of 69.50 mg/kg and had no significant effect on biochemical, hematological, and histological parameters.
CONCLUSION
The results suggest that the stem bark extract of possesses antimalarial activity.
背景
在喀麦隆传统医学中用于治疗疟疾及相关症状。然而，其抗疟原虫功效仍有待确定。
目的
本研究旨在评估[植物名称]提取物的抗疟原虫活性及口服急性毒性。
材料与方法
通过将[植物名称]的叶和茎皮在蒸馏水、甲醇、乙醇、二氯甲烷（DCM）和己烷中浸渍来制备提取物。所有提取物最初针对耐氯喹的恶性疟原虫W2株进行筛选以确认其抗疟活性，并且对最有效的提取物在疟疾小鼠模型中以三种浓度（100、200和400mg/kg体重）进行评估。还测定了生化、血液学和组织学参数。
结果
总体而言，7种提取物显示出抗疟原虫活性，IC50范围为0.809μg/mL至5.886μg/mL。[植物名称]茎皮的水提取物最有效（IC50 = 0.809μg/mL），并进一步评估了其对感染疟原虫的小鼠的急性毒性和疗效。该提取物对小鼠安全，其半数致死剂量（LD50）高于2000mg/kg体重。它还具有良好的抗疟疗效，ED50为69.50mg/kg，并且对生化、血液学和组织学参数没有显著影响。
结论
结果表明[植物名称]茎皮提取物具有抗疟活性。
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