Kim YoonYi, Noh Chanyoung, Yu Myungheon, Bae Minji, Jo Kyubong
Department of Chemistry, Sogang University, Seoul 04107, Korea.
Chem Commun (Camb). 2023 Jul 27;59(61):9388-9391. doi: 10.1039/d3cc02112b.
Nanopore sequencing maps biochemical processes on DNA by detecting negative peaks in the sequence alignment profile. Protein-bound DNA and single-strand broken DNA cannot pass through nanopores, resulting in unaligned regions in the genome MAP. This novel approach provides a clear representation of genomic biochemical events.
纳米孔测序通过检测序列比对图谱中的负峰来绘制DNA上的生化过程。与蛋白质结合的DNA和单链断裂的DNA无法通过纳米孔，导致基因组图谱中出现未比对区域。这种新方法清晰地呈现了基因组生化事件。
