用于绘制DNA分子上生化过程的负性纳米孔测序

Negative nanopore sequencing for mapping biochemical processes on DNA molecules.

作者信息

Kim YoonYi, Noh Chanyoung, Yu Myungheon, Bae Minji, Jo Kyubong

机构信息

Department of Chemistry, Sogang University, Seoul 04107, Korea.

出版信息

Chem Commun (Camb). 2023 Jul 27;59(61):9388-9391. doi: 10.1039/d3cc02112b.

DOI:10.1039/d3cc02112b

PMID:37435665

Abstract

Nanopore sequencing maps biochemical processes on DNA by detecting negative peaks in the sequence alignment profile. Protein-bound DNA and single-strand broken DNA cannot pass through nanopores, resulting in unaligned regions in the genome MAP. This novel approach provides a clear representation of genomic biochemical events.

摘要

纳米孔测序通过检测序列比对图谱中的负峰来绘制DNA上的生化过程。与蛋白质结合的DNA和单链断裂的DNA无法通过纳米孔，导致基因组图谱中出现未比对区域。这种新方法清晰地呈现了基因组生化事件。