鼠伤寒沙门氏菌突变体通用转导的快速方法。
Rapid methods for generalized transduction of Salmonella typhimurium mutants.
作者信息
Rosenfeld S A, Brenchley J
出版信息
J Bacteriol. 1979 Apr;138(1):261-3. doi: 10.1128/jb.138.1.261-263.1979.
Abstract
A procedure has been developed that allows the propagation of generalized transducing phage directly on cells growing on solid media. After the donor cells are killed with chloroform, the phage can be transferred directly to recipient cells and transductants can be selected.
摘要
已开发出一种方法，可使广义转导噬菌体直接在固体培养基上生长的细胞上进行增殖。在用氯仿杀死供体细胞后，噬菌体可直接转移至受体细胞，并可筛选出转导子。
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